ISSN: 1449-2288International Journal of Biological Sciences
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Int J Biol Sci 2010; 6(7):649-654. doi:10.7150/ijbs.6.649 This issue Cite
Short Research Communication
Mechanical Forces Used for Cell Fractionation Can Create Hybrid Membrane Vesicles
The Netherlands Cancer Institute, Division of Cell Biology. Amsterdam, the Netherlands.
Citation:
Salomon I, Janssen H, Neefjes J. Mechanical Forces Used for Cell Fractionation Can Create Hybrid Membrane Vesicles. Int J Biol Sci 2010; 6(7):649-654. doi:10.7150/ijbs.6.649. https://www.ijbs.com/v06p0649.htm
Other stylesAbstract
The ability to understand the inner works of the cell requires methods for separation of intracellular membrane-enclosed compartments. Disruption of the plasma membrane (PM) by mechanical forces to investigate the content of the cell is common practice. Whether vesicles or membranes of different sources can fuse as a result is unclear. If such contamination occurs, conclusions based on these techniques should consider these. Utilizing an endoplasmic reticulum (ER) membrane marker and a PM marker, we were able to detect the source of membranes following the breakup of cells using flow cytometry and immuno Electron Microscopy (immuno EM). Fractionation processes produced a small fraction of new membrane entities from two distinctively different origins generated during the initial disruption steps in a temperature independent manner, stressing that defining organelles or intrinsic fusion events based on such procedures and markers are valid when exceeding the small number of vesciles fused during the fractionation process.
Keywords: membrane fusion, fractionation, trafficking, signalling, proteomics.
Citation styles
APA
Salomon, I., Janssen, H., Neefjes, J. (2010). Mechanical Forces Used for Cell Fractionation Can Create Hybrid Membrane Vesicles. International Journal of Biological Sciences, 6(7), 649-654. https://doi.org/10.7150/ijbs.6.649.
ACS
Salomon, I.; Janssen, H.; Neefjes, J. Mechanical Forces Used for Cell Fractionation Can Create Hybrid Membrane Vesicles. Int. J. Biol. Sci. 2010, 6 (7), 649-654. DOI: 10.7150/ijbs.6.649.
NLM
Salomon I, Janssen H, Neefjes J. Mechanical Forces Used for Cell Fractionation Can Create Hybrid Membrane Vesicles. Int J Biol Sci 2010; 6(7):649-654. doi:10.7150/ijbs.6.649. https://www.ijbs.com/v06p0649.htm
CSE
Salomon I, Janssen H, Neefjes J. 2010. Mechanical Forces Used for Cell Fractionation Can Create Hybrid Membrane Vesicles. Int J Biol Sci. 6(7):649-654.
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