Int J Biol Sci 2011; 7(5):607-617. doi:10.7150/ijbs.7.607 This issue Cite

Research Paper

Immunophenotyping at the Time of Diagnosis Distinguishes Two Groups of Nasopharyngeal Carcinoma Patients: Implications for Adoptive Immunotherapy

Jiang Li1,2,3*, Qiu-yan Chen4*, Haoyuan Mo4, Yi-lan Zhang1,2,3, Zhou-feng Huang1,2,3, Yi-xin Zeng1,2,3 ✉

1. State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou, China;
2. Department of Experimental Research, Sun Yat-sen University Cancer Center, Guangzhou, China;
3. Department of Biotherapy, Sun Yat-sen University Cancer Center, Guangzhou, China;
4. Department of Nasopharyngeal Carcinoma, Sun Yat-sen University Cancer Center, Guangzhou, China.
* Co-first author

Citation:
Li J, Chen Qy, Mo H, Zhang Yl, Huang Zf, Zeng Yx. Immunophenotyping at the Time of Diagnosis Distinguishes Two Groups of Nasopharyngeal Carcinoma Patients: Implications for Adoptive Immunotherapy. Int J Biol Sci 2011; 7(5):607-617. doi:10.7150/ijbs.7.607. https://www.ijbs.com/v07p0607.htm
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Abstract

Background: Adoptive immunotherapy with EBV-specific CTLs (EBV-CTL) has been used to treat EBV-associated nasopharyngeal carcinoma (NPC) but only a fraction of the patients shows noticeable clinical response.

Patients and Methods: Sixty-seven newly diagnosed NPC patients from 2005 to 2007 and 21 healthy donors were collected. Immunological parameters and immune function of PBMCs and EBV-CTL were analyzed by flow cytometer analysis (FACS) and 51Cr releasing experiment; Molecular characteristics on NPC tumor cells were investigated by immunochemical staining and statistic analysis.

Results: NPC patients can be classified into two groups based on the percentage of CD3+ T cells in peripheral blood before accepted any treatment, (>52.6%, mean-2SE from healthy controls, NPC Group 1; <52.6%, NPC Group 2). The patients in Group 2 showed a significant decrease of CD3+CD8+ T-cells, CD3+CD4+ T-cells and CD3+CD45RO+ memory T cells, and increase of CD3-CD16+ NK cells compared to Group 1 patients and healthy controls (P<0.001). EBV-specific T cell responses, were weaker in this group of patients and their tumor cells expressed lower levels of the EBV encoded latent membrane protein (LMP)-1 and HLA class II protein compared with the patients of NPC Group 1 (P<0.05) .

Conclusion: These findings demonstrate that NPC patients could be distinguished on the basis of their immune status which will affect the efficacy of EBV-CTL immunotherapy.

Keywords: NPC, EBV-specific CTL, immunotherapy, LMP1, immunophenotype


Citation styles

APA
Li, J., Chen, Q.y., Mo, H., Zhang, Y.l., Huang, Z.f., Zeng, Y.x. (2011). Immunophenotyping at the Time of Diagnosis Distinguishes Two Groups of Nasopharyngeal Carcinoma Patients: Implications for Adoptive Immunotherapy. International Journal of Biological Sciences, 7(5), 607-617. https://doi.org/10.7150/ijbs.7.607.

ACS
Li, J.; Chen, Q.y.; Mo, H.; Zhang, Y.l.; Huang, Z.f.; Zeng, Y.x. Immunophenotyping at the Time of Diagnosis Distinguishes Two Groups of Nasopharyngeal Carcinoma Patients: Implications for Adoptive Immunotherapy. Int. J. Biol. Sci. 2011, 7 (5), 607-617. DOI: 10.7150/ijbs.7.607.

NLM
Li J, Chen Qy, Mo H, Zhang Yl, Huang Zf, Zeng Yx. Immunophenotyping at the Time of Diagnosis Distinguishes Two Groups of Nasopharyngeal Carcinoma Patients: Implications for Adoptive Immunotherapy. Int J Biol Sci 2011; 7(5):607-617. doi:10.7150/ijbs.7.607. https://www.ijbs.com/v07p0607.htm

CSE
Li J, Chen Qy, Mo H, Zhang Yl, Huang Zf, Zeng Yx. 2011. Immunophenotyping at the Time of Diagnosis Distinguishes Two Groups of Nasopharyngeal Carcinoma Patients: Implications for Adoptive Immunotherapy. Int J Biol Sci. 7(5):607-617.

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