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Int J Biol Sci 2012; 8(10):1335-1344. doi:10.7150/ijbs.4654 This issue Cite

Research Paper

Stem Cell Transplantation Increases Antioxidant Effects in Diabetic Mice

Ming Li ¹, Luca Vanella ², Yuming Zhang ³, Ming Shi ¹, Takashi Takaki⁴, Joseph I Shapiro², Susumu Ikehara¹

1. Department of Stem Cell Disorders, Kansai Medical University, Moriguchi City, Osaka, Japan.
2. School of Medicine, University of Marshall, 1600 Medical Center Drive, Huntington, WV 25701, USA.
3. Department of Pediatrics, Nanfang Hospital, Guangzhou, China.
4. JEOL Ltd., Tokyo, Japan.

✉ Corresponding author: Susumu Ikehara, M.D., Ph.D., Department of Stem Cell Disorders, Kansai Medical University, Moriguchi City, Osaka 570-8506, Japan. Telephone: 81-6-6993-9625; Fax: 81-6-6994-9627; e-mail: ikeharakmu.ac.jp.

Abstract

Intra bone marrow-bone marrow transplantation (IBM- BMT) + thymus transplantation (TT) has been shown to reduce the incidence of graft versus host disease (GVHD) and restore donor-derived T cell function. In addition, an increase in insulin sensitivity occurred in db/db mice after IBM-BMT+TT treatment. Heme oxygenase (HO)-1 is a stress inducible enzyme which exert antioxidant, antiapoptotic, and immune-modulating properties. We examined whether IBM-BMT+TT could modulate the expression of HO-1 in the kidneys of db/db mice. Six-week-old db/db mice with blood glucose levels higher than 250 mg/dl were treated with IBM-BMT+TT. Six weeks later, the db/db mice showed decreased body weight, blood glucose levels and insulin, and increased plasma adiponectin levels. The upregulation of HO-1 was associated with significantly (p<0.05) increased levels of peNOS and pAKT, but decreased levels of iNOS in the kidneys of db/db mice. Plasma creatinine levels also decreased (p<0.05), and the expression of type IV collagen was improved. Thus IBM-BMT+TT unregulated the expression of HO-1, peNOS and pAKT, while decreasing iNOS levels in the kidney of db/db mice. This was associated with an improvement in renal function.

Keywords: IBM-BMT+TT, antioxidant, HO-1, diabetic nephropathy.

Introduction

Heme oxygenase (HO)-1 is a stress inducible enzyme that catalyzes the degradation of heme proteins into free iron, CO and biliverdin, which is then rapidly converted into bilirubin. These catabolic end products exert antioxidant, antiapoptotic, and immune-modulating properties, rendering the overall function of HO-1 to be cytoprotective [1, 2]. Graft versus host disease (GVHD) is a major side effect of allogenic bone marrow transplantation (BMT) [3]. Intra bone marrow- BMT (IBM-BMT) appears to offer the best approach for allogenic BMT, as manifested by a reduced incidence of GVHD and the restoration of donor-derived T cell function [4-6]. We recently reported that, in db/db mice treated with IBM-BMT+ thymus (TT) transplantation, CD4/CD8 ratios were normalized with a consequent increase in plasma adiponectin levels and insulin sensitivity. In addition, the expression of pancreatic phosphorylated AK transforming factor (pAKT), phosphorylated liver kinase B1 (pLKB1), phosphorylated adenosine monophosphate-activated protein kinase (pAMPK) and HO-1 increased in thus-treated db/db mice [7].

The db/db mouse presents with renal abnormalities including proteinuria, glomerular hypertrophy, and glomerulosclerosis [8, 9], thereby making it an ideal animal model in which to study renal and vascular dysfunction in diabetic nephropathy (DN). DN is the major cause of end-stage renal failure, mainly due to the increased incidence of type 2 diabetes mellitus [10]. Oxidative stress (OS) is considered a significant pathogenic factor in the development of diabetic vascular complications, including nephropathy [11, 12]. The beneficial effect of increased HO-1 expression and HO activity further suggests that the abnormality in endothelial progenitor cells is due to a mesenchymal stem cell (MSC) disorder exacerbated by OS and decreased levels of adiponectin [13].

In the present study, we examined whether allogenic IBM-BMT+TT modulated the expression of HO-1 in the kidneys of db/db mice and show that this intervention does indeed upregulate the expression of HO-1, phosphorylated endothelial nitric oxide synthase (peNOS) and pAKT, decrease inducible NOS (iNOS), and improve renal function, therefore providing a mechanistic basis for the therapeutic use of IBM-BMT+TT.

Materials and methods

Animals

Six-, 12- and 30-wk-old BKS.Cg-m+Lepr^db/+Lepr^db/J (H-2K^d) (db/db) mice and age-matched lean mice were purchased from Charles River Laboratories (Yokohama, Japan) and maintained in animal facilities under specific pathogen-free conditions. All procedures were performed under protocols approved by the Institutional Animal Care and Use Committee at Kansai Medical University. Body weight and blood glucose levels were measured each week. Blood glucose levels higher than 250 mg/dl on two consecutive measurements were considered to indicate the onset of diabetes. Each experiment was repeated three times.

IBM-BMT+TT

Six-wk-old db/db mice with blood glucose levels greater than 250 mg/dl and proteinuria greater than ++ on two consecutive measurements received fractionated irradiation twice a day (5.0 Gy×2, 4-hour interval). One day after irradiation, whole BMCs from B6 mice were injected into the recipient mice (1×10⁷/mouse) by IBM-BMT using our previously-described method [7]. Simultaneously, the thymus from newborn B6 mice was grafted under the renal capsule of the left kidney of the recipient mice. All treated mice were sacrificed 6 wks after IBM-BMT+TT treatment.

Immunochemistry and measurement of nitric oxide (NO) levels

The pancreata, livers, kidneys and bones of lean and db mice were removed at each time point. After the tissues were fixed in 10% formalin for 24 hours at room temperature, they were embedded in paraffin. The sections (3-μm thick) were stained with hematoxylin and eosin. The pancreata were stained with polyclonal guinea pig anti-swine insulin antibody (N1542, Dako Cytomation, CA). The kidneys were stained with collagen type IV (Sigma-Aldrich, MO), pAKT, iNOS and eNOS (Abcam PLC, Cambridge, UK) antibodies. The stained sections were examined under a microscope.

NO levels in the kidneys were measured according to the manufacturer's protocol using a Nitric Oxide Assay Kit (BioAssay Systems, Hayward, CA).

Electron microscopy

The fixed renal cortices were embedded in epoxy resin using conventional methods [14]. Kidney tissue was fixed in 2.5% glutaraldehyde in 0.1M PBS, pH 7.4. Samples were washed and post fixed with 2% aqueous OsO₄. The samples were then dehydrated with serially-increasing concentrations of ethanol (60 to 100%), and were infiltrated with epon mixed with methyl nadic anhydride (TAAB Laboratories, UK), Dodecenyl succinic anhydride (TAAB Laboratories), and Quetol-812 and DMP-30 (Nishin Company, Japan). Ultrathin sections were prepared, stained using uranyl acetate and lead citrate, and then examined with a JEM-1400A electron microscope (JEOL, Tokyo, Japan). The measurements were taken from electron micrographs with magnifications of x2,500 and x12,000.

Adiponectin, insulin, creatinine and pancreatic insulin content measurements

Adiponectin was determined in mouse plasma using an ELISA assay (R&D Systems, Inc. MN and Invitrogen Corporation CA). Insulin was measured using an ELISA kit (Morinaga, Yokohama, Japan).

Plasma creatinine was measured according to the manufacturer's protocols using a plasma creatinine detection kit (Arbor Assays, Michigan).

For insulin content measurement, the pancreas was isolated and weighed, and then placed in 3ml cold acid ethanol (0.18M HCl in 70% ethanol) and homogenated. This was kept overnight at 4°C and centrifuged at 2400rpm for 30min at 4°C. The supernatant was transferred and stored at -20°C for measurement using ELISA.

Western blot analysis of kidney HO-1, peNOS, AKT, pAKT, and iNOS

At sacrifice, kidneys were dissected, then pooled for each mouse in order to measure signaling molecules. Specimens were stored at -140°C until assayed. Frozen kidney tissues were pulverized under liquid nitrogen and placed in a homogenization buffer (mmol/l: 10 phosphate buffer, 250 sucrose, 1 EDTA, 0.1 PMSF and 0.1% v/v tergitol, pH 7.5). Homogenates were centrifuged at 27,000 g for 10 min at 4ºC, and the supernatant was then isolated and protein levels were obtained by immunoblotting with the relevant antibodies. Antibodies against HO-1, AKT, phosphorylated AKT (pAKT), eNOS, peNOS and iNOS were obtained from Cell Signaling Technology, Inc. (Beverly, MA). Antibodies were prepared by dilution as described previously [15, 16].

Flow cytometric analyses

Peripheral blood mononuclear cells were obtained from the tail vein of mice 30 days after transplantation. These cells were stained with antibodies against PE-H-2K^d, PE-CD4, PE-CD8a and FITC-H-2K^b (BD Bioscience Pharmingen, San Diego, CA) for 30 min on ice. After washing twice with 2% FCS / PBS and lysing red blood cells, the 10000 events acquired were analyzed by FACScan (BD Bioscience). Isotype-matched immunoglobulins were used as controls.

Statistical analysis

Statistical significance between experimental groups was determined by the Fisher method of analysis of multiple comparisons. For comparison between treatment groups, the null hypothesis was tested by either a single-factor ANOVA for multiple groups or unpaired t test for two groups. Statistical significance was regarded as significant at p<0.05.

Results

Body weight, thymus weight, and morphology of pancreas and bone, and insulin content of pancreas islet

We first confirmed the parameters (body weight, thymus weight and morphology of pancreas and bone marrow) of the db/db mice and lean mice (as control) maintained in our animal facilities (Fig.1), since food and environment may affect some parameters of db/db mice. As seen in Fig. 1A, the body weight of 12-wk-old lean mice was significantly higher than that of 6-wk-old lean mice (30.4 ±0.5 vs 22.6±0.1 g, p< 0.001). Similar results occurred in the db/db mice (53.2 ±1.7 vs 33.5±0.03 g, p< 0.001). However, although the body weight of 30-wk-old db/db mice was significantly higher than that of 12-wk-old db/db mice (61.2±0.6 vs 53.2± 1.7 g, p< 0.01), there was no significant difference in body weight between 30-wk-old and 12-wk-old lean mice (32.4±0.5 vs 30.4± 0.5 g). As shown in Fig. 1B, thymus weight was significantly lower in the 6-wk-, 12-wk and 30-wk-old db/db mice than in the age-matched lean mice (51.7 ±4.7 vs 75.7±3.3, 21.3 ±0.9 vs 49±4.0, 20.5 ±1.2 vs 43±4.1mg, p < 0.05 at each time point).

On HE staining, 12-wk- and 30-wk-old db/db mice (Fig. 1E and F) exhibited larger islets than 6 wk-old db/db mice and lean mice (Fig. 1C and D). More adipocytes were present in the bone marrow of 12-wk- and 30-wk-old db/db mice (Fig. 1H-J) than in lean mice (Fig. 1G).

The db/db mouse shows insulin resistance and insulin-positive cell failure. The insulin content (brown color) was much lower in the larger islets of 6-wk-, 12-wk- and 30-wk-old db/db mice (Fig. 1L-N) than in those of lean mice (Fig. 1K), suggesting that more insulin was secreted into the peripheral blood in the db/db mice. Pancreatic insulin contents of lean mice (2336±84.5 pg/ml per mg pancreas), and 6-wk- (1149±37.5 pg/ml per pancreas), 12-wk- (521±21.7 pg/ml per mg pancreas), and 30-wk- (180±21.3 pg/ml per mg pancreas) old db/db mice were measured by ELISA.

Plasma creatinine levels and histology of the kidneys of db/db mice

As shown in Fig. 1O, plasma creatinine levels were significantly higher in 12-wk-old when compared to 6-wk-old db/db mice (3.41±0.20 vs 1.47±0.46 mg/dl, p<0.05), and in 30-wk-old compared to 12-wk-old db/db mice (4.65±0.03 vs 3.41±0.20 mg/dl, p<0.05). Electron microscopic images of the kidneys are shown in Fig. 1P-S. 12-wk and 30-wk db/db mice exhibited severe podocyte foot fusion. Electron dense deposits and increased numbers of mesangium cells were observed in the 30-wk-old db/db mice (Fig. 1R and S).

 Figure 1 

Body weight, thymus weight and the morphology of pancreas and bone marrow, and expression of insulin on the pancreata, and plasma creatinine levels and images of electron microcopy. (A) Body weights are shown (*p< 0.01). (B) Thymus weights are shown (*p<0.05). (C-J) HE staining of pancreas and bone marrow of the lean and db/db mice at each age. The islets are shown (* in Fig.C-F). The adipocytes are shown in db/db mice (arrows in Fig. H-J). (K-N) Immunochemistry staining for insulin (arrows) was performed. Scale bar =50μm in Fig. C-J, Scale bar =25μm in Fig. K-N. (O) Plasma creatinine levels of lean, (p< 0.05). (P-S) EM of kidneys. Podocyte foot fusion is shown in Fig. 3Q and R (arrows). Electron dense deposits and increased mesangium cells (*) are shown in Fig. 1R and S. Scale bar =2μm in Fig. P-R, Scale bar =10μm in Fig. S. The results are mean±SE, n=6 in each group.

Improved body weight, blood glucose and plasma adiponectin, and insulin levels 6 weeks after treatment with IBM-BMT +TT

As seen in Fig.2A, body weight was significantly (p<0.01) lower in the IBM-BMT+TT treated db/db mice than in the age-matched non-treated db/db mice. In addition, fasting blood glucose levels (Fig. 2B) were significantly (p<0.01) lower in the treated db/db mice than in the non-treated db/db mice. Indeed IBM-BMT+TT reduced blood glucose to the levels found in age-matched lean mice. As shown in Fig. 2C, the non-treated db/db mice exhibited a significantly (p<0.05) lower level of plasma adiponectin than age-matched lean mice. However, the plasma adiponectin levels were significantly (p<0.05) increased in the treated db/db mice when compared to the non-treated db/db mice (Fig. 2C). Plasma insulin levels were higher (p<0.05) in the non-treated db/db mice than in the age matched lean mice. IBM-BMT+TT treatment resulted in a decrease (p<0.05) in plasma insulin levels to the levels found in age-matched lean animals (Fig. 2D).

Lymphocyte subpopulations in peripheral blood and cell number of thymus 6 weeks after treatment with IBM-BMT +TT

The relative percentage of the total cell number in the thymus was significantly (p<0.05) lower in the non-treated db/db mice than in the lean mice (65.13% of that of lean mice). The total cell number of the thymus was significantly higher (p<0.05) increased in the treated mice (97.9% of lean mice) (Fig. 3A). Figure 3B shows the percentages of donor-derived cells (H-2K^b) in the peripheral blood of the treated db/db mice. The percentages of donor-derived CD4⁺ and CD8⁺ were 10.16% and 7.81% respectively, Figure 3C and D.

 Figure 2 

Body weight, blood glucose levels, plasma adiponectin, insulin. (A) Body weights are shown. (B) Blood glucose levels are shown. (C) Plasma adiponectin. (D) Plasma insulin.

 Figure 3 

Cell number of thymus and lymphocyte subpopulations in peripheral blood. (A) The relative percentage of total cell numbers in the thymus, which was significantly increased in the db/db mice treated with IBM-BMT+TT. (B-D) Donor-derived cells in peripheral blood, CD4⁺ cells and CD8⁺ cells are shown. The results are mean±SE, n=6 in each group.

Lymphocyte function

Approximately 98% of hematolymphoid cells were of donor-origin in the peripheral blood of the recipients one month after IBM-BMT+TT treatment. Spleen cells of the recipient animals demonstrated mitogen responses to lipopolysaccharide, in contrast to those of non-treated db/db mice: 26296±2780.93 vs 19292±1310.42, respectively, p<0.05. In lean mice the mitogen response was 31406±2780.93. These findings suggest that IBM-BMT+TT treatment restores lymphocyte function in the db/db mice.

Expression of HO-1, iNOS, peNOS and pAKT in the kidney of db/db mice treated with IBM-BMT+TT

The expression of renal HO-1 was significantly lower (p<0.05) in the non-treated db/db mice when compared to lean mice, but significantly increased (p<0.05) in db/db mice treated with IBM-BMT+TT, when compared with non-treated db/db mice (Fig. 4A and B). The expression of iNOS was the receptacle of HO-1 expression and was significantly (p<0.05) higher in the non-treated db/db mice than in the lean mice. Densitometry analyses revealed a significant decrease in iNOS (p<0.05) in the kidney of treated db/db mice when compared with non-treated db/db mice (Fig. 4A and C).

The expression of pAKT and peNOS in the kidney was significantly lower (p<0.05) in the non-treated db/db mice when compared to the lean mice, but there was a significant increase in the expression of both pAKT and peNOS in the IBM-BMT+TT treated db/db mice, when compared with non-treated db/db mice (Fig. 4D-G, p<0.05). The expression of type IV collagen (brown color) was greater in the kidney of the non-treated db/db mice (Fig. 4I) than in the lean mice (Fig. 4H). However, it was significantly lower in the kidney (arrows in Fig. 4J) in the treated db/db mice than in the non-treated db/db mice.

 Figure 4 

Expression of HO-1, iNOS AKT, pAKT, eNOS and peNOS in the kidney, and expression of type IV collagen in the kidneys. (A-C) Western blot and densitometry analysis of HO-1, iNOS and actin proteins in kidneys of lean, non-treated db/db mice and treated db/db mice. (D and E) Western blot and densitometry analyses of AKT and pAKT and actin proteins in kidney of lean, non-treated db/db mice and treated db/db mice are shown. (F and G) Western blot and densitometry analyses of eNOS and peNOS and actin proteins in kidney of lean, non-treated db/db mice and treated db/db mice are shown. Representative immunoblots are shown. There were significant differences between non-treated and treated groups (p<0.05). (H-J) Immunochemistry staining for type IV collagen (*). (K-S) Immunochemistry staining for eNOS, pAKT and iNOS. Scale bar =25μm. (T) NO levels in the kidney.

The expression of peNOS, pAKT and iNOS was confirmed by immunochemistry staining (Fig. 4K-T). eNOS positive cells were identified in the kidneys of lean, non-treated and treated db/db mice ( arrow in Fig. 4K-M). pAKT positive cells were identified in the kidney of lean and treated db/db mice (arrow in Fig. 4N and P). However, none were found in the non-treated db/db mice. iNOS positive cells were identified in the kidney of non-treated db/db mice (arrow and *in Fig. 4R).

As shown in Fig. 4T, NO levels in the kidney were significantly lower in non-treated db/db mice when compared to lean mice (0.35±0.004 vs 0.03±0.002 μM/mg, p<0.01). However, the NO levels were significantly higher in the kidney (0.09±0.005 vs 0.03±0.002 μM/mg, p<0.01) in the treated db/db mice than in the non-treated db/db mice.

Discussion

Leptin receptor-deficient db/db mice exhibit severe hereditary obesity [17] and display hormonal imbalances and hematolymphoid defects [18, 19]. db/db mice exhibit a marked reduction in the size and cellularity of the thymus [20, 21]. Thus we firstly confirmed the body weight and thymus weight of db/db and lean mice at each age that were maintained in our animal facilities. Meantime, the morphology of the pancreas, kidney and bone marrow was also confirmed (Figure 1- 3). There were significant differences in body weight and thymus weight between 6-wk-and 12-wk-old mice. The pancreas, kidney and bone marrow showed differences in morphology at each time point. Previous studies have shown that IBM-BMT+TT improved insulin sensitivity in db/db mice 7 wks after treatment [7]. However, there are no reports indicating whether IBM-BMT+TT increased HO-1 expression, or benefited the kidney in db/db mice, although a protective effect of BMT on renal pathology has been reported [22].

HO-1 is the inducible form of HO (HO-2 is the constitute form) and is increased in response to OS. Increased levels of HO-1 slow the rate of weight gain and decrease levels of TNF-α and IL-6, but increase serum levels of adiponectin in obese diabetic mice [15]. db/db mice treated with IBM-BMT+TT exhibited a larger decrease in body weight and plasma insulin levels than age-matched non-treated db/db mice. Insulin administration to treated db/db mice produced a rapid decrease in blood glucose levels, suggesting improved insulin sensitivity after IBM-BMT+TT treatment. In the present study, HO-1 expression increased in the kidney after IBM-BMT+TT and was accompanied by an enhanced expression of peNOS and pAKT. A reduction in the production of nitric oxide [23] and a decrease in the expression of eNOS are reportedly associated with advanced DN [24]. An increase in AKT signaling is considered an important metabolic response to OS through the attenuation of ROS-mediated endothelial dysfunction [25]. The up regulation of renal HO-1 expression with a resultant increase in signaling molecules, including adiponectin via the pAKT-pAMPK-peNOS pathway, prevents the development of metabolic syndrome and improves both vascular and renal function [26].

HO has been shown to be important for attenuating the overall production of reactive oxygen species (ROS) through its ability to degrade heme and to produce carbon monoxide (CO), biliverdin/bilirubin, and the release of free iron, which possess potent antioxidant properties and antiapoptotic effects [26]. Recently, the induction of HO-1 has been found to reduce diabetic induced-glomerular injury and apoptosis, and these effects are associated with decreased NF-κB-induced inflammation and oxidative stress [27]. MSCs have the capacity to repair renal injury, accelerate tubular proliferation and improve renal function, and upregulate HO-1 expression and increase HO activity, all are essential for MSC growth and differentiation to the osteoblast lineage, which is consistent with the role of HO-1 in hematopoietic stem cell differentiation [28]. Induction of HO-1 by cobalt-protoporphyrin IX in recipient mice before conditioning and bone marrow transplantation (BMT) results in a reduction of GVHD and improved survival [29]. One report has indicated that HO-1 in dendritic cells may function as an inhibitor of the alloimmune response mediated by CD4+ T cells. HO-1 could thus play a key role in the design of therapies to prolong allograft function, based on the potent alloimmunity modulating capacity of this enzyme [30].

Plasma adiponectin levels are also significantly higher in treated db/db mice than in non-treated db/db mice. Adiponectin is a key regulator of albuminuria, suppressing not only i) OS and albuminuria but also ii) high levels of adiponectin phosphorylase and iii) active AMPK, presumably via adipoR1, which prevents OS and the fusion of the podocyte foot processes [31]. There is a report supporting the hypothesis that adiponectin is critical for both endothelial cell survival and function via the activation of eNOS and crosstalk between pAKT and pAMPK [32].

Bone marrow cells have the capacity to differentiate into mesangial cells [33, 34] and to transdifferentiate into podocytes. This is accompanied by the re-expression of the defective collagen chains and improved renal histology and function [35]. MSCs have the capacity to repair renal injury, accelerate tubular proliferation and improve renal function [36, 37]. Nephrons are of mesenchymal origin and stroma cells are of crucial importance for signaling, leading to the differentiation of both nephrons and collecting ducts [38]. Bone marrow-derived mesangial cell progenitors may play a crucial role in the development and progression of extracellular matrix accumulation and mesangial cell proliferation in the db/db mouse. Future studies to clarify extra-cellular matrix accumulation will focus on the donor-derived BM cells in the kidneys of the recipient.

Type IV collagen is a major structural component of all basement membranes, including the glomerular basement membrane of the kidney in vertebrates and invertebrates [39, 40]. Our results show that the expression of type IV collagen in the glomerulus was attenuated after IBM-BMT+TT. BM-derived cells fuse with existing glomerular cells and thereby provide therapeutic benefit or, alternatively, transfer their nuclei to damaged podocytes and thereby enable repair. Bone marrow-derived stem cells repair basement membrane collagen defects and reverse genetic kidney disease [35].

Age-related hematologic changes are associated with a decline in BM cellularity and a decline in adaptive immunity [41, 42]. The thymus involutes steadily with increasing age, resulting in a decreased release of new naïve T cells to the periphery, thereby affecting adaptive immunity [43]. The thymus also undergoes age-related progressive involution with decreased thymic lymphopoiesis, reduced thymic size and disrupted thymic architecture. Our previous studies demonstrated that the thymus is significantly lighter in db/db mice than in age-matched lean mice. When thymi from newborn C57BL6 mice were transplanted into db/db mice, the percentage of double-positive, double-negative and CD4+ cells in the thymus was normalized, as was the CD4/CD8 ratio in the peripheral blood [7], and the total cell number of the thymus, suggesting that IBM-BMT+TT is capable of restoring the immune repertoire and overcoming the autoimmune response, which is considered to be partly responsible for the development of diabetes.

IBM-BMT appears to be the most appropriate strategy for allogenic BMT [4]. Moreover, allogenic IBM-BMT+TT normalizes T cell subsets, cytokine imbalance and insulin sensitivity in db/db mice [7]. Allogenic IBM-BMT+TT upregulates the expression of HO-1 in kidney. This is followed by the upregulation of peNOS and pAKT and a reduction in iNOS levels, resulting in an improvement in renal function. This therapeutic approach offers decided advantages in the treatment of both autoimmune and hematological diseases.

Supplementary Material

 Supplementary Figure 1 

Abbreviations

DN: diabetic nephropathy; GVHD: Graft versus host disease; HO: Heme oxygenase; IBM-BMT: Intra bone marrow-bone marrow transplantation; iNOS: inducible nitric oxide synthase; NO: Nitric oxide; OS: Oxidative stress; pAKT: phosphorylated AK transforming; peNOS: phosphorylated endothelial nitric oxide synthase; pLKB1: phosphorylated liver kinase B1; pAMPK: phosphorylated adenosine monophosphate-activated protein kinase; TT: thymus transplantation.

Acknowledgements

We would like to thank Mr. Hilary Eastwick-Field and Ms. Keiko Ando for their help in the preparation of the manuscript. This study was supported by Otsuka Pharmaceutical Company, Ltd and by NIH grants DK068234, HL55601, HL34300 (NGA). We thank Ms Aiko Kitajima for her excellent technical assistance regarding the morphology studies.

Competing Interests

The authors have declared that no competing interest exists.

References

1. Otterbein LE, Soares MP, Yamashita K, Bach FH. Heme oxygenase-1: unleashing the protective properties of heme. Trends Immunol. 2003;24:449-55

2. Orozco LD, Kapturczak MH, Barajas B, Wang X, Weinstein MM, Wong J. et al. Heme oxygenase-1 expression in macrophages plays a beneficial role in atherosclerosis. Circ Res. 2007;100:1703-11

3. Goulmy E, Schipper R, Pool J, Blokland E, Falkenburg JH, Vossen J. et al. Mismatches of minor histocompatibility antigens between HLA-identical donors and recipients and the development of graft-versus-host disease after bone marrow transplantation. N Engl J Med. 1996;334:281-5

4. Ikehara S. A novel strategy for allogeneic stem cell transplantation: perfusion method plus intra-bone marrow injection of stem cells. Exp Hematol. 2003;31:1142-6

5. Nakamura K, Inaba M, Sugiura K, Yoshimura T, Kwon AH, Kamiyama Y. et al. Enhancement of allogeneic hematopoietic stem cell engraftment and prevention of GVHD by intra-bone marrow bone marrow transplantation plus donor lymphocyte infusion. Stem Cells. 2004;22:125-34

6. Hosaka N, Nose M, Kyogoku M, Nagata N, Miyashima S, Good RA. et al. Thymus transplantation, a critical factor for correction of autoimmune disease in aging MRL/+mice. Proc Natl Acad Sci U S A. 1996;93:8558-62

7. Li M, Abraham NG, Vanella L, Zhang Y, Inaba M, Hosaka N. et al. Successful modulation of type 2 diabetes in db/db mice with intra-bone marrow--bone marrow transplantation plus concurrent thymic transplantation. J Autoimmun. 2010;35:414-23

8. Breyer MD, Bottinger E, Brosius FC 3rd, Coffman TM, Harris RC, Heilig CW. et al. Mouse models of diabetic nephropathy. J Am Soc Nephrol. 2005;16:27-45

9. Ziyadeh FN, Hoffman BB, Han DC, Iglesias-De La Cruz MC, Hong SW, Isono M. et al. Long-term prevention of renal insufficiency, excess matrix gene expression, and glomerular mesangial matrix expansion by treatment with monoclonal antitransforming growth factor-beta antibody in db/db diabetic mice. Proc Natl Acad Sci U S A. 2000;97:8015-20

10. Ritz E, Tarng DC. Renal disease in type 2 diabetes. Nephrol Dial Transplant. 2001;16(Suppl 5):11-8

11. Baynes JW. Role of oxidative stress in development of complications in diabetes. Diabetes. 1991;40:405-12

12. Kakimoto M, Inoguchi T, Sonta T, Yu HY, Imamura M, Etoh T. et al. Accumulation of 8-hydroxy-2'-deoxyguanosine and mitochondrial DNA deletion in kidney of diabetic rats. Diabetes. 2002;51:1588-95

13. Abraham NG, Li M, Vanella L, Peterson SJ, Ikehara S, Asprinio D. Bone marrow stem cell transplant into intra-bone cavity prevents type 2 diabetes: role of heme oxygenase-adiponectin. J Autoimmun. 2008;30:128-35

14. Toki J, Adachi Y, Jin T, Fan T, Takase K, Lian Z. et al. Enhancement of IL-7 following irradiation of fetal thymus. Immunobiology. 2003;207:247-58

15. Li M, Kim DH, Tsenovoy PL, Peterson SJ, Rezzani R, Rodella LF. et al. Treatment of obese diabetic mice with a heme oxygenase inducer reduces visceral and subcutaneous adiposity, increases adiponectin levels, and improves insulin sensitivity and glucose tolerance. Diabetes. 2008;57:1526-35

16. Peterson SJ, Kim DH, Li M, Positano V, Vanella L, Rodella LF. et al. The L-4F mimetic peptide prevents insulin resistance through increased levels of HO-1, pAMPK, and pAKT in obese mice. J Lipid Res. 2009;50:1293-304

17. Chua SC Jr, Chung WK, Wu-Peng XS, Zhang Y, Liu SM, Tartaglia L. et al. Phenotypes of mouse diabetes and rat fatty due to mutations in the OB (leptin) receptor. Science. 1996;271:994-6

18. Fantuzzi G, Faggioni R. Leptin in the regulation of immunity, inflammation, and hematopoiesis. J Leukoc Biol. 2000;68:437-46

19. Matarese G, Moschos S, Mantzoros CS. Leptin in immunology. J Immunol. 2005;174:3137-42

20. Fernandes G, Handwerger BS, Yunis EJ, Brown DM. Immune response in the mutant diabetic C57BL/Ks-dt+ mouse. Discrepancies between in vitro and in vivo immunological assays. J Clin Invest. 1978;61:243-50

21. Kimura M, Tanaka S, Isoda F, Sekigawa K, Yamakawa T, Sekihara H. T lymphopenia in obese diabetic (db/db) mice is non-selective and thymus independent. Life Sci. 1998;62:1243-50

22. Flaquer M, Franquesa M, Barquinero J, Lloberas N, Gutierrez C, Torras J. et al. Bone marrow transplantation induces normoglycemia in a type 2 diabetes mellitus murine model. Transplant Proc. 2009;41:2282-5

23. Amiri F, Shaw S, Wang X, Tang J, Waller JL, Eaton DC. et al. Angiotensin II activation of the JAK/STAT pathway in mesangial cells is altered by high glucose. Kidney Int. 2002;61:1605-16

24. Marrero MB, Banes-Berceli AK, Stern DM, Eaton DC. Role of the JAK/STAT signaling pathway in diabetic nephropathy. Am J Physiol Renal Physiol. 2006;290:F762-8

25. Schulz E, Dopheide J, Schuhmacher S, Thomas SR, Chen K, Daiber A. et al. Suppression of the JNK pathway by induction of a metabolic stress response prevents vascular injury and dysfunction. Circulation. 2008;118:1347-57

26. Abraham NG, Cao J, Sacerdoti D, Li X, Drummond G. Heme oxygenase: the key to renal function regulation. Am J Physiol Renal Physiol. 2009;297:F1137-52

27. Elmarakby AA, Faulkner J, Baban B, Saleh MA, Sullivan JC. Induction of hemeoxygenase-1 reduces glomerular injury and apoptosis in diabetic spontaneously hypertensive rats. Am J Physiol Renal Physiol. 2012;302:F791-800

28. Vanella L, Sanford C Jr, Kim DH, Abraham NG, Ebraheim N. Oxidative stress and heme oxygenase-1 regulated human mesenchymal stem cells differentiation. Int J Hypertens. 2012;2012:890671

29. Gerbitz A, Ewing P, Wilke A, Schubert T, Eissner G, Dietl B. et al. Induction of heme oxygenase-1 before conditioning results in improved survival and reduced graft-versus-host disease after experimental allogeneic bone marrow transplantation. Biol Blood Marrow Transplant. 2004;10:461-72

30. Cheng C, Noorderloos M, van Deel ED, Tempel D, den Dekker W, Wagtmans K. et al. Dendritic cell function in transplantation arteriosclerosis is regulated by heme oxygenase 1. Circ Res. 2010;106:1656-66

31. Sharma K, Ramachandrarao S, Qiu G, Usui HK, Zhu Y, Dunn SR. et al. Adiponectin regulates albuminuria and podocyte function in mice. J Clin Invest. 2008;118:1645-56

32. Ouchi N, Kobayashi H, Kihara S, Kumada M, Sato K, Inoue T. et al. Adiponectin stimulates angiogenesis by promoting cross-talk between AMP-activated protein kinase and Akt signaling in endothelial cells. J Biol Chem. 2004;279:1304-9

33. Ito T, Suzuki A, Imai E, Okabe M, Hori M. Bone marrow is a reservoir of repopulating mesangial cells during glomerular remodeling. J Am Soc Nephrol. 2001;12:2625-35

34. Wong CY, Cheong SK, Mok PL, Leong CF. Differentiation of human mesenchymal stem cells into mesangial cells in post-glomerular injury murine model. Pathology. 2008;40:52-7

35. Sugimoto H, Mundel TM, Sund M, Xie L, Cosgrove D, Kalluri R. Bone-marrow-derived stem cells repair basement membrane collagen defects and reverse genetic kidney disease. Proc Natl Acad Sci U S A. 2006;103:7321-6

36. Lange C, Togel F, Ittrich H, Clayton F, Nolte-Ernsting C, Zander AR. et al. Administered mesenchymal stem cells enhance recovery from ischemia/reperfusion-induced acute renal failure in rats. Kidney Int. 2005;68:1613-7

37. Morigi M, Imberti B, Zoja C, Corna D, Tomasoni S, Abbate M. et al. Mesenchymal stem cells are renotropic, helping to repair the kidney and improve function in acute renal failure. J Am Soc Nephrol. 2004;15:1794-804

38. Anglani F, Forino M, Del Prete D, Tosetto E, Torregrossa R, D'Angelo A. In search of adult renal stem cells. J Cell Mol Med. 2004;8:474-87

39. Kalluri R, Shield CF, Todd P, Hudson BG, Neilson EG. Isoform switching of type IV collagen is developmentally arrested in X-linked Alport syndrome leading to increased susceptibility of renal basement membranes to endoproteolysis. J Clin Invest. 1997;99:2470-8

40. Hudson BG, Tryggvason K, Sundaramoorthy M, Neilson EG. Alport's syndrome, Goodpasture's syndrome, and type IV collagen. N Engl J Med. 2003;348:2543-56

41. Hakim FT, Gress RE. Immunosenescence: deficits in adaptive immunity in the elderly. Tissue Antigens. 2007;70:179-89

42. Linton PJ, Dorshkind K. Age-related changes in lymphocyte development and function. Nat Immunol. 2004;5:133-9

43. Aspinall R. Age-associated thymic atrophy in the mouse is due to a deficiency affecting rearrangement of the TCR during intrathymic T cell development. J Immunol. 1997;158:3037-45

Author contact

Corresponding author: Susumu Ikehara, M.D., Ph.D., Department of Stem Cell Disorders, Kansai Medical University, Moriguchi City, Osaka 570-8506, Japan. Telephone: 81-6-6993-9625; Fax: 81-6-6994-9627; e-mail: ikeharakmu.ac.jp.

Received 2012-5-26
Accepted 2012-9-13
Published 2012-10-27

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