Int J Biol Sci 2015; 11(6):643-651. doi:10.7150/ijbs.10785 This issue Cite

Research Paper

Garlic Oil Suppressed Nitrosodiethylamine-Induced Hepatocarcinoma in Rats by Inhibiting PI3K-AKT-NF-κB Pathway

Cui-Li Zhang, Tao Zeng, Xiu-Lan Zhao, Ke-Qin Xie

Institute of Toxicology, School of Public Health, Shandong University, 44 Wenhua West Road, Jinan 250012, Shandong, P.R. China.

Citation:
Zhang CL, Zeng T, Zhao XL, Xie KQ. Garlic Oil Suppressed Nitrosodiethylamine-Induced Hepatocarcinoma in Rats by Inhibiting PI3K-AKT-NF-κB Pathway. Int J Biol Sci 2015; 11(6):643-651. doi:10.7150/ijbs.10785. https://www.ijbs.com/v11p0643.htm
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Abstract

Graphic abstract

To explore the underlying mechanisms for the protective effects of garlic oil (GO) against nitrosodiethylamine (NDEA)-induced hepatocarcinoma, 60 male Wistar rats were randomized into 4 groups (n=15): control group, NDEA group, and two GO plus NDEA groups. The rats in GO plus NDEA groups were pretreated with GO (20 or 40 mg/kg) for 7 days. Then, all rats except those in control group were gavaged with NDEA for 20 weeks, and the rats in GO plus NDEA groups were continuously administered with GO. The results showed that GO co-treatment significantly suppressed the NDEA-induced increases of alpha fetal protein (AFP) level in serum, nuclear atypia in H&E staining, sirius red-positive areas and proliferating cell nuclear antigen (PCNA) expression. The molecular mechanisms exploration revealed that the protein levels of phosphatidylinositol 3 kinase (PI3K)-p85, PI3K-p110, total AKT, p-AKT (Ser473) and p-AKT (Thr308) in the liver of NDEA group rats were higher than those in control group rats. In addition, NDEA treatment induced IκB degradation and NF-κB p65 phosphorylation, and up-regulated the protein levels of downstream pro-inflammatory mediators. GO co-treatment significantly reversed all the above adverse effects induced by NDEA. These results suggested that the protective effects of GO against NDEA-induced hepatocarcinoma might be associated with the suppression of PI3K- AKT-NF-κB pathway.

Keywords: Garlic oil, Nitrosodiethylamine, Hepatocarcinoma, Phosphatidylinositol 3 kinase (PI3K), AKT, NF-κB


Citation styles

APA
Zhang, C.L., Zeng, T., Zhao, X.L., Xie, K.Q. (2015). Garlic Oil Suppressed Nitrosodiethylamine-Induced Hepatocarcinoma in Rats by Inhibiting PI3K-AKT-NF-κB Pathway. International Journal of Biological Sciences, 11(6), 643-651. https://doi.org/10.7150/ijbs.10785.

ACS
Zhang, C.L.; Zeng, T.; Zhao, X.L.; Xie, K.Q. Garlic Oil Suppressed Nitrosodiethylamine-Induced Hepatocarcinoma in Rats by Inhibiting PI3K-AKT-NF-κB Pathway. Int. J. Biol. Sci. 2015, 11 (6), 643-651. DOI: 10.7150/ijbs.10785.

NLM
Zhang CL, Zeng T, Zhao XL, Xie KQ. Garlic Oil Suppressed Nitrosodiethylamine-Induced Hepatocarcinoma in Rats by Inhibiting PI3K-AKT-NF-κB Pathway. Int J Biol Sci 2015; 11(6):643-651. doi:10.7150/ijbs.10785. https://www.ijbs.com/v11p0643.htm

CSE
Zhang CL, Zeng T, Zhao XL, Xie KQ. 2015. Garlic Oil Suppressed Nitrosodiethylamine-Induced Hepatocarcinoma in Rats by Inhibiting PI3K-AKT-NF-κB Pathway. Int J Biol Sci. 11(6):643-651.

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