Int J Biol Sci 2016; 12(1):42-52. doi:10.7150/ijbs.12275 This issue

Research Paper

Apigenin-7-O-β-D-(-6''-p-coumaroyl)-Glucopyranoside Treatment Elicits Neuroprotective Effect against Experimental Ischemic Stroke

Min Cai1,*, Yulong Ma2,*, Wei Zhang3,*, Shiquan Wang2,*, Ying Wang1, Li Tian2, Zhengwu Peng1, Huaning Wang1, Tan Qingrong1✉

1. Department of Psychiatry;
2. Department of Anesthesiology;
3. Department of Pharmacology; Xijing hospital, the Forth Military Medical School
* These authors contributed equally to this work.

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Cai M, Ma Y, Zhang W, Wang S, Wang Y, Tian L, Peng Z, Wang H, Qingrong T. Apigenin-7-O-β-D-(-6''-p-coumaroyl)-Glucopyranoside Treatment Elicits Neuroprotective Effect against Experimental Ischemic Stroke. Int J Biol Sci 2016; 12(1):42-52. doi:10.7150/ijbs.12275. Available from

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Graphic abstract

Stroke is the major cause of permanent disability and mortality in China. Apigenin-7-O-β-D-(-6''-p-coumaroyl)-glucopyranoside (APG) is a glycoside subtype of apigenin and has the antioxidant activity; however, whether and how it plays a neuroprotective role following cerebral ischemia remains unknown. In present study, we adopted the oxygen glucose/reperfusion model in PC12 cells, bilateral common carotid artery occlusion model in C57B6 mice and middle cerebral artery occlusion model in SD rats to observe the therapeutic effects of APG on ischemic stroke. We also discussed the underlying mechanism. Treatment with 0.4 μg/ml or 0.8 μg/ml APG promoted cell viability and proliferation, reduced LDH release and apoptotic cell death levels in PC12 cells. Treatment with 50 mg/kg or 100 mg/kg APG at 30 minutes after reperfusion improved neurological outcomes in vivo, as demonstrated by elevation of neurological scores in both mice and rats. It also increased the number of survival neurons in mice and reduced infarct volume in rats. APG also increased the contents of Mn-SOD and the phosphorylation level of STAT3, elevated the antioxidant activity and reduced oxidative productions. These findings revealed a neuroprotective effect of APG, which possibly induced by the STAT3 phosphorylation-mediated Mn-SOD up-regulation.

Keywords: Apigenin-7-O-β-D-(-6''-p-coumaroyl)-glucopyranoside (APG), cerebral ischemia/reperfusion, oxidative stress, manganese-superoxide dismutase