Andrographolide Inhibits Angiogenesis by Inhibiting the Mir-21-5p/TIMP3 Signaling Pathway

Dai, Jianwei; Lin, Yuyin; Duan, Youfa; Li, Zixuan; Zhou, Dalei; Chen, Wensheng; Wang, Lijing; Zhang, Qian-Qian

doi:10.7150/ijbs.19194

Theranostics

International Journal of Medical Sciences

Nanotheranostics

Journal of Cancer

Journal of Genomics

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Int J Biol Sci 2017; 13(5):660-668. doi:10.7150/ijbs.19194 This issue Cite

Research Paper

Andrographolide Inhibits Angiogenesis by Inhibiting the Mir-21-5p/TIMP3 Signaling Pathway

Jianwei Dai^1*, Yuyin Lin^1*, Youfa Duan^2*, Zixuan Li², Dalei Zhou², Wensheng Chen¹, Lijing Wang^2✉, Qian-Qian Zhang^2✉

1. GMU-GIBH Joint School of Life Sciences, Guangzhou Medical University, Guangzhou 510000, China;
2. Vascular Biology Research Institute, School of Basic Course, Guangdong Pharmaceutical University, Guangzhou 510006, China.
* These authors contributed equally to this work.

Citation:

Dai J, Lin Y, Duan Y, Li Z, Zhou D, Chen W, Wang L, Zhang QQ. Andrographolide Inhibits Angiogenesis by Inhibiting the Mir-21-5p/TIMP3 Signaling Pathway. Int J Biol Sci 2017; 13(5):660-668. doi:10.7150/ijbs.19194. https://www.ijbs.com/v13p0660.htm

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Abstract

Angiogenesis provides nutrients and oxygen to promote tumor growth and affords a channel that facilitates tumor cell entry into the circulation. Andrographolide (Andro) possess anti-tumor activity; however, its direct effect on angiogenesis still needs to be clarified. In this study, our experiments revealed that Andro significantly inhibited vascular growth in chick embryo chorioallantoic membrane (CAM) and yolk sac membrane (YSM) models. Meanwhile, tumor angiogenesis was also suppressed by Andro. Additionally, we found that cell proliferation, migration and tube formation of vascular endothelial cells was inhibited by Andro treatment in vitro. The effect was primarily mediated through inhibition of miR-21-5p expression and further targeting of TIMP3. This work provides evidence that Andro directly inhibits angiogenesis and might be an effective anti-angiogenic therapeutic drug for cancer treatment.

Keywords: Andrographolide, angiogenesis, miR-21-5p, TIMP3.

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APA

Dai, J., Lin, Y., Duan, Y., Li, Z., Zhou, D., Chen, W., Wang, L., Zhang, Q.Q. (2017). Andrographolide Inhibits Angiogenesis by Inhibiting the Mir-21-5p/TIMP3 Signaling Pathway. International Journal of Biological Sciences, 13(5), 660-668. https://doi.org/10.7150/ijbs.19194.

ACS

Dai, J.; Lin, Y.; Duan, Y.; Li, Z.; Zhou, D.; Chen, W.; Wang, L.; Zhang, Q.Q. Andrographolide Inhibits Angiogenesis by Inhibiting the Mir-21-5p/TIMP3 Signaling Pathway. Int. J. Biol. Sci. 2017, 13 (5), 660-668. DOI: 10.7150/ijbs.19194.

NLM

CSE

Dai J, Lin Y, Duan Y, Li Z, Zhou D, Chen W, Wang L, Zhang QQ. 2017. Andrographolide Inhibits Angiogenesis by Inhibiting the Mir-21-5p/TIMP3 Signaling Pathway. Int J Biol Sci. 13(5):660-668.

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