Int J Biol Sci 2017; 13(8):961-975. doi:10.7150/ijbs.20074 This issue

Research Paper

Vasopressin Mediates the Renal Damage Induced by Limited Fructose Rehydration in Recurrently Dehydrated Rats

Fernando E. García-Arroyo1, Edilia Tapia1,2, Mónica G Blas-Marron1, Guillermo Gonzaga1, Octaviano Silverio1, Magdalena Cristóbal1,2, Horacio Osorio1,2, Abraham S. Arellano-Buendía1,2, Cecilia Zazueta3, Omar Emiliano Aparicio-Trejo4, Juan G. Reyes-García5, José Pedraza-Chaverri4, Virgilia Soto6, Carlos Roncal-Jiménez7, Richard J. Johnson7, Laura G Sánchez-Lozada1,2✉

1. Laboratory of Renal Physiopathology. INC Ignacio Chávez. Mexico City. Mexico
2. Dept. of Nephrology. INC Ignacio Chávez. Mexico City. Mexico
3. Dept. of Cardiovascular Biomedicine. INC Ignacio Chávez. Mexico City. Mexico
4. Dept. of Biology. Faculty of Chemistry, UNAM. Mexico City. Mexico
5. Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, IPN Mexico City. Mexico
6. Dept. of Pathology. INC Ignacio Chávez. Mexico City. Mexico
7. Div. of Renal Diseases, University of Colorado, Aurora CO, USA

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license ( See for full terms and conditions.
García-Arroyo FE, Tapia E, Blas-Marron MG, Gonzaga G, Silverio O, Cristóbal M, Osorio H, Arellano-Buendía AS, Zazueta C, Aparicio-Trejo OE, Reyes-García JG, Pedraza-Chaverri J, Soto V, Roncal-Jiménez C, Johnson RJ, Sánchez-Lozada LG. Vasopressin Mediates the Renal Damage Induced by Limited Fructose Rehydration in Recurrently Dehydrated Rats. Int J Biol Sci 2017; 13(8):961-975. doi:10.7150/ijbs.20074. Available from

File import instruction


Graphic abstract

Recurrent dehydration and heat stress cause chronic kidney damage in experimental animals. The injury is exacerbated by rehydration with fructose-containing beverages. Fructose may amplify dehydration-induced injury by directly stimulating vasopressin release and also by acting as a substrate for the aldose reductase-fructokinase pathway, as both of these systems are active during dehydration. The role of vasopressin in heat stress associated injury has not to date been explored. Here we show that the amplification of renal damage mediated by fructose in thermal dehydration is mediated by vasopressin. Fructose rehydration markedly enhanced vasopressin (copeptin) levels and activation of the aldose reductase-fructokinase pathway in the kidney. Moreover, the amplification of the renal functional changes (decreased creatinine clearance and tubular injury with systemic inflammation, renal oxidative stress, and mitochondrial dysfunction) were prevented by the blockade of V1a and V2 vasopressin receptors with conivaptan. On the other hand, there are also other operative mechanisms when water is used as rehydration fluid that produce milder renal damage that is not fully corrected by vasopressin blockade. Therefore, we clearly showed evidence of the cross-talk between fructose, even at small doses, and vasopressin that interact to amplify the renal damage induced by dehydration. These data may be relevant for heat stress nephropathy as well as for other renal pathologies due to the current generalized consumption of fructose and deficient hydration habits.

Keywords: Vasopressin, chronic kidney disease