CTGF Contributes to the Development of Posterior Capsule Opacification: an <i>in vitro</i> and <i>in vivo</i> study

Ma, Bo; Jing, Ruihua; Liu, Jie; Yang, Lan; Li, Jingming; Qin, Li; Cui, Lijun; Pei, Cheng

doi:10.7150/ijbs.23946

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Int J Biol Sci 2018; 14(4):437-448. doi:10.7150/ijbs.23946 This issue Cite

Research Paper

CTGF Contributes to the Development of Posterior Capsule Opacification: an in vitro and in vivo study

Bo Ma^1*, Ruihua Jing^1*, Jie Liu¹, Lan Yang², Jingming Li¹, Li Qin¹, Lijun Cui¹, Cheng Pei^1✉

1. Department of Ophthalmology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
2. Ningbo Medical Center Lihuili Eastern Hospital, Ningbo, Zhejiang, China.
^*Co-first authors

Citation:

Ma B, Jing R, Liu J, Yang L, Li J, Qin L, Cui L, Pei C. CTGF Contributes to the Development of Posterior Capsule Opacification: an in vitro and in vivo study. Int J Biol Sci 2018; 14(4):437-448. doi:10.7150/ijbs.23946. https://www.ijbs.com/v14p0437.htm

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Abstract

Connective tissue growth factor (CTGF) is a crucial factor that plays a major role in the process of posterior capsule opacification (PCO). However, the effects of CTGF on the proliferation and migration of lens epithelial cells (LECs) and on the mechanism of the epithelial mesenchymal transition (EMT) and extracellular matrix (ECM) in human lens epithelial cells (HLECs) as well as the effects of shRNA-mediated CTGF knockdown on the development of PCO in rats remain unclear. In the present study, we found that CTGF promoted EMT, proliferation, migration and the expression of p-ERK1/2 protein in HLECs but exerted little effect on the expression of p-p38 and p-JNK1/2 proteins. MEK inhibitor U0126 effectively restrained the CTGF-induced expression of α-smooth muscle actin (α-SMA), fibronectin (Fn) and type I collagen (COL-1) in HLECs. CTGF knockdown effectively postponed the onset of PCO in the rats and significantly reduced the expression of α-SMA in the capsule. In conclusion, CTGF contributed to the development of PCO presumably by promoting proliferation, migration of LECs, EMT specific protein expression and ECM synthesis in HLECs, which is dependent on ERK signalling. Furthermore, blocking CTGF effectively inhibited PCO in the rats and the EMT specific protein expression in the lens capsule.

Keywords: CTGF, Posterior Capsule Opacification, Epithelial Mesenchymal Transition, Extracellular Matrix, ERK signalling

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APA

Ma, B., Jing, R., Liu, J., Yang, L., Li, J., Qin, L., Cui, L., Pei, C. (2018). CTGF Contributes to the Development of Posterior Capsule Opacification: an in vitro and in vivo study. International Journal of Biological Sciences, 14(4), 437-448. https://doi.org/10.7150/ijbs.23946.

ACS

Ma, B.; Jing, R.; Liu, J.; Yang, L.; Li, J.; Qin, L.; Cui, L.; Pei, C. CTGF Contributes to the Development of Posterior Capsule Opacification: an in vitro and in vivo study. Int. J. Biol. Sci. 2018, 14 (4), 437-448. DOI: 10.7150/ijbs.23946.

NLM

CSE

Ma B, Jing R, Liu J, Yang L, Li J, Qin L, Cui L, Pei C. 2018. CTGF Contributes to the Development of Posterior Capsule Opacification: an in vitro and in vivo study. Int J Biol Sci. 14(4):437-448.

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