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Int J Biol Sci 2019; 15(1):158-168. doi:10.7150/ijbs.28392 This issue Cite

Research Paper

Exosomes Derived from Human Induced Pluripotent Stem Cells-Endothelia Cells Promotes Postnatal Angiogenesis in Mice Bearing Ischemic Limbs

Meng Ye*, Qihong Ni*, Haozhe Qi*, Xin Qian, Jiaquan Chen, Xiangjiang Guo, Maoran Li, Yiping Zhao, Guanhua Xue, Haoyu Deng✉, Lan Zhang✉

Department of Vascular Surgery, RenJi Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China
*These authors equally contribute to this work.

✉ Corresponding authors: Lan Zhang, Department of Vascular Surgery, RenJi Hospital, Shanghai Jiaotong Unviersity School of Medicine, Shanghai, China, 200127, Email: rjzhanglanedu.cn, Phone:(+86)189-1835-7825, Fax: (021)6838-5664 and Haoyu Deng, More

Citation:
Ye M, Ni Q, Qi H, Qian X, Chen J, Guo X, Li M, Zhao Y, Xue G, Deng H, Zhang L. Exosomes Derived from Human Induced Pluripotent Stem Cells-Endothelia Cells Promotes Postnatal Angiogenesis in Mice Bearing Ischemic Limbs. Int J Biol Sci 2019; 15(1):158-168. doi:10.7150/ijbs.28392. https://www.ijbs.com/v15p0158.htm
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Abstract

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Induced pluripotent stem cell (iPSC) derived endothelial cells (ECs) is a novel therapeutic option for ischemic diseases. Although the detailed mechanism of this novel therapy remains unknown, emerging evidence has demonstrated that exosomes derived from hiPSC-ECs play a critical role in this approach. In this study, we first isolated and characterized the exosomes from iPSCs-ECs (hiPSC-EC-Exo) and determined the functional roles of hiPSC-EC-Exo in neovascularization and the underlying mechanism. Further, we evaluated the effect of exosomes derived from hiPS-ECs on promoting angiogenesis in a mouse model bearing ischemic limbs. Our results showed that miR-199b-5p, an miRNA highly associated with angiogenesis, is significantly upregulated during the differentiation of hiPSC-ECs. Mechanically, our studies found that hiPSC-ECs expressing miR-199b-5p significantly promote cell migration, proliferation and tube formation through Jagged-1-dependent upregulation of VEGFR2 in HUVECs. Similarly, coculture of hiPSC-ECs-Exo with HUVECs also resulted in a significant improvement in HUVEC migration, proliferation, and tube formation, suggesting that exosome-mediated cell-cell communication in a paracrine manner may serve as a fundamental mechanism for iPSC-EC-based treatment. Consequently, we found that the transfer of hiPSC-ECs enriched with miR-199b-5p significantly enhanced micro-vessel density and blood perfusion in ischemic limbs in vivo. Taken together, our studies were the first to demonstrate that transfer of hiPSC-ECs-Exo is a promising approach to treat ischemic injury via the mechanism of promoting neovascularization.

Keywords: hiPSC-EC, exosome, angiogenesis, Jagged-1, miR-199b-5p

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APA
Ye, M., Ni, Q., Qi, H., Qian, X., Chen, J., Guo, X., Li, M., Zhao, Y., Xue, G., Deng, H., Zhang, L. (2019). Exosomes Derived from Human Induced Pluripotent Stem Cells-Endothelia Cells Promotes Postnatal Angiogenesis in Mice Bearing Ischemic Limbs. International Journal of Biological Sciences, 15(1), 158-168. https://doi.org/10.7150/ijbs.28392.

ACS
Ye, M.; Ni, Q.; Qi, H.; Qian, X.; Chen, J.; Guo, X.; Li, M.; Zhao, Y.; Xue, G.; Deng, H.; Zhang, L. Exosomes Derived from Human Induced Pluripotent Stem Cells-Endothelia Cells Promotes Postnatal Angiogenesis in Mice Bearing Ischemic Limbs. Int. J. Biol. Sci. 2019, 15 (1), 158-168. DOI: 10.7150/ijbs.28392.

NLM
Ye M, Ni Q, Qi H, Qian X, Chen J, Guo X, Li M, Zhao Y, Xue G, Deng H, Zhang L. Exosomes Derived from Human Induced Pluripotent Stem Cells-Endothelia Cells Promotes Postnatal Angiogenesis in Mice Bearing Ischemic Limbs. Int J Biol Sci 2019; 15(1):158-168. doi:10.7150/ijbs.28392. https://www.ijbs.com/v15p0158.htm

CSE
Ye M, Ni Q, Qi H, Qian X, Chen J, Guo X, Li M, Zhao Y, Xue G, Deng H, Zhang L. 2019. Exosomes Derived from Human Induced Pluripotent Stem Cells-Endothelia Cells Promotes Postnatal Angiogenesis in Mice Bearing Ischemic Limbs. Int J Biol Sci. 15(1):158-168.

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