miR-197-3p Represses the Proliferation of Prostate Cancer by Regulating the VDAC1/AKT/β-catenin Signaling Axis

Huang, Qiang; Ma, Bo; Su, Yixi; Chan, Kawo; Qu, Hu; Huang, Jiayu; Wang, Dejuan; Qiu, Jianguang; Liu, Huanliang; Yang, Xiangling; Wang, Zhongyang

doi:10.7150/ijbs.42019

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Theranostics

International Journal of Medical Sciences

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Journal of Cancer

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Int J Biol Sci 2020; 16(8):1417-1426. doi:10.7150/ijbs.42019 This issue Cite

Research Paper

miR-197-3p Represses the Proliferation of Prostate Cancer by Regulating the VDAC1/AKT/β-catenin Signaling Axis

Qiang Huang¹, Bo Ma¹, Yixi Su^2,3, Kawo Chan², Hu Qu¹, Jiayu Huang¹, Dejuan Wang¹, Jianguang Qiu¹, Huanliang Liu^2,3, Xiangling Yang², Zhongyang Wang^1✉

1. Department of Urology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China
2. Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, Guangdong Institute of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China
3. Department of Clinical Laboratory, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China

Citation:

Huang Q, Ma B, Su Y, Chan K, Qu H, Huang J, Wang D, Qiu J, Liu H, Yang X, Wang Z. miR-197-3p Represses the Proliferation of Prostate Cancer by Regulating the VDAC1/AKT/β-catenin Signaling Axis. Int J Biol Sci 2020; 16(8):1417-1426. doi:10.7150/ijbs.42019. https://www.ijbs.com/v16p1417.htm

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Abstract

Accumulating investigations have demonstrated that microRNAs (miRNAs) are promising efficient targets for the next generation of molecular therapeutics. The development of miRNA-based therapies requires the identification and validation of cancer-associated miRNAs. Herein, we identified that miR-197-3p regulates the carcinogenesis and development of prostate cancer (PCa) via bioinformatics analysis. Next, we investigated the function and regulatory mechanisms of miR-197-3p in PCa. Overexpression of miR-197-3p suppressed PCa cell proliferation and colony formation. In contrast, inhibition of miR-197-3p activity enhanced PCa cell proliferation and colony formation. Mechanistic investigations identified that voltage dependent anion channel 1 (VDAC1) is a direct target of miR-197-3p. miR-197-3p targeting of VDAC1 resulted in downregulation of p-Akt and β-catenin. Subsequently, we found that restoration of VDAC1 abolished the effects of miR-197-3p on PCa cell proliferation and AKT signaling pathway. Furthermore, we confirmed that miR-197-3p suppressed tumor xenograft growth in vivo. In conclusion, our study offers an empirical investigation of miR-197-3p, a tumor suppressor that may be a potential therapeutic target in PCa.

Keywords: miR-197-3p, prostate cancer, VDAC1, AKT

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APA

Huang, Q., Ma, B., Su, Y., Chan, K., Qu, H., Huang, J., Wang, D., Qiu, J., Liu, H., Yang, X., Wang, Z. (2020). miR-197-3p Represses the Proliferation of Prostate Cancer by Regulating the VDAC1/AKT/β-catenin Signaling Axis. International Journal of Biological Sciences, 16(8), 1417-1426. https://doi.org/10.7150/ijbs.42019.

ACS

Huang, Q.; Ma, B.; Su, Y.; Chan, K.; Qu, H.; Huang, J.; Wang, D.; Qiu, J.; Liu, H.; Yang, X.; Wang, Z. miR-197-3p Represses the Proliferation of Prostate Cancer by Regulating the VDAC1/AKT/β-catenin Signaling Axis. Int. J. Biol. Sci. 2020, 16 (8), 1417-1426. DOI: 10.7150/ijbs.42019.

NLM

CSE

Huang Q, Ma B, Su Y, Chan K, Qu H, Huang J, Wang D, Qiu J, Liu H, Yang X, Wang Z. 2020. miR-197-3p Represses the Proliferation of Prostate Cancer by Regulating the VDAC1/AKT/β-catenin Signaling Axis. Int J Biol Sci. 16(8):1417-1426.

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