The Novel Role of hnRNP UL1 in Human Cell Nucleoli

Cichocka, Marlena; Karlik, Anna; Plewka, Patrycja; Gawade, Kishor; Stępień, Agata; Świergiel, Patrycja; Gadgil, Ankur; Raczyńska, Katarzyna Dorota

doi:10.7150/ijbs.75084

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Int J Biol Sci 2022; 18(13):4809-4823. doi:10.7150/ijbs.75084 This issue Cite

Research Paper

The Novel Role of hnRNP UL1 in Human Cell Nucleoli

Marlena Cichocka^1,2, Anna Karlik^1,2, Patrycja Plewka^1,2, Kishor Gawade^1,2, Agata Stępień¹, Patrycja Świergiel^1,2, Ankur Gadgil^1,2, Katarzyna Dorota Raczyńska^1,2✉

1. Laboratory of RNA Processing, Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Uniwersytetu Poznańskiego 6, 61-614 Poznan, Poland
2. Center for Advanced Technology, Adam Mickiewicz University, Uniwersytetu Poznańskiego 10, 61-614 Poznan, Poland

Citation:

Cichocka M, Karlik A, Plewka P, Gawade K, Stępień A, Świergiel P, Gadgil A, Raczyńska KD. The Novel Role of hnRNP UL1 in Human Cell Nucleoli. Int J Biol Sci 2022; 18(13):4809-4823. doi:10.7150/ijbs.75084. https://www.ijbs.com/v18p4809.htm

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Abstract

hnRNP UL1 plays an important role in cell nuclei, where it is recruited to DNA damage sites and is involved in the repair of DNA double-strand breaks. Furthermore, this protein is known as a transcriptional repressor of RNA polymerase II genes. In the present study, we have shown that hnRNP UL1 is also localized in the nucleoli of human cells. Upon investigating its function, we found that hnRNP UL1 stimulates ribosomal DNA (rDNA) gene transcription. Moreover, we observed that cells with hnRNP UL1 silencing exhibited increased sensitivity to DNA damage. We also showed that hnRNP UL1 interacts with γH2A.X, RPA32, XRCC1, and Chk1 in cell nucleoli, suggesting its involvement in the repair of rDNA damage.

Keywords: hnRNP UL1, nucleolus, rDNA genes, transcription, rDNA damage, rDNA repair

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APA

Cichocka, M., Karlik, A., Plewka, P., Gawade, K., Stępień, A., Świergiel, P., Gadgil, A., Raczyńska, K.D. (2022). The Novel Role of hnRNP UL1 in Human Cell Nucleoli. International Journal of Biological Sciences, 18(13), 4809-4823. https://doi.org/10.7150/ijbs.75084.

ACS

Cichocka, M.; Karlik, A.; Plewka, P.; Gawade, K.; Stępień, A.; Świergiel, P.; Gadgil, A.; Raczyńska, K.D. The Novel Role of hnRNP UL1 in Human Cell Nucleoli. Int. J. Biol. Sci. 2022, 18 (13), 4809-4823. DOI: 10.7150/ijbs.75084.

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CSE

Cichocka M, Karlik A, Plewka P, Gawade K, Stępień A, Świergiel P, Gadgil A, Raczyńska KD. 2022. The Novel Role of hnRNP UL1 in Human Cell Nucleoli. Int J Biol Sci. 18(13):4809-4823.

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