1. Department of Experimental Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, Italy. 2. Department of Environmental, Biological, Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, 81100 Caserta, Italy.
✉ Corresponding author: Prof. Rosanna Chianese, Department of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Via Costantinopoli 16, 80138, Napoli, Italy. Tel. Number: +39 081 5667528; E-mail: rosanna.chianeseit.
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Manfrevola F, Potenza N, Chioccarelli T, Di Palo A, Siniscalchi C, Porreca V, Scialla A, Mele VG, Petito G, Russo A, Lanni A, Senese R, Ricci G, Pierantoni R, Chianese R, Cobellis G. Actin remodeling driven by circLIMA1: sperm cell as an intriguing cellular model. Int J Biol Sci 2022; 18(13):5136-5153. doi:10.7150/ijbs.76261. https://www.ijbs.com/v18p5136.htm
CircRNA cargo in spermatozoa (SPZ) participates in setting cell quality, in terms of morphology and motility. Cannabinoid receptor CB1 activity is correlated with a proper spermatogenesis and epididymal sperm maturation. Despite CB1 promotes endogenous skill to circularize mRNAs in SPZ, few notions are reported regarding the functional link between endocannabinoids and spermatic circRNA cargo. In CB1 knock-out male mice, we performed a complete dataset of spermatic circRNA content by microarray strategy.
Differentially expressed (DE)-circRNAs, as a function of genotype, were identified. Within DE-circRNAs, we focused the attention on circLIMA1, as putative actin-cytoskeleton architecture regulator. The validation of circLIMA1 dependent-competitive endogenous RNA (ceRNA) network (ceRNET) in in vitro cell line confirmed its activity in the regulation of the cytoskeletal actin. Interestingly, a dynamic actin regulation in SPZ nuclei was found during their epididymal maturation.
In this scenario, we showed for the first time an intriguing sperm nuclear actin remodeling, regulated via a ceRNET-independent pathway, consisting in the nuclear shuttling of circLIMA1-QKI interactome and downstream in Gelsolin regulation. In particular, the increased levels of circLIMA1 in CB1 knock-out SPZ, associated with an inefficient depolymerization of nuclear actin, specifically illustrate how endocannabinoids, by regulating circRNA cargo, may contribute to sperm morpho-cellular maturation.
Manfrevola, F., Potenza, N., Chioccarelli, T., Di Palo, A., Siniscalchi, C., Porreca, V., Scialla, A., Mele, V.G., Petito, G., Russo, A., Lanni, A., Senese, R., Ricci, G., Pierantoni, R., Chianese, R., Cobellis, G. (2022). Actin remodeling driven by circLIMA1: sperm cell as an intriguing cellular model. International Journal of Biological Sciences, 18(13), 5136-5153. https://doi.org/10.7150/ijbs.76261.
ACS
Manfrevola, F.; Potenza, N.; Chioccarelli, T.; Di Palo, A.; Siniscalchi, C.; Porreca, V.; Scialla, A.; Mele, V.G.; Petito, G.; Russo, A.; Lanni, A.; Senese, R.; Ricci, G.; Pierantoni, R.; Chianese, R.; Cobellis, G. Actin remodeling driven by circLIMA1: sperm cell as an intriguing cellular model. Int. J. Biol. Sci. 2022, 18 (13), 5136-5153. DOI: 10.7150/ijbs.76261.
NLM
Manfrevola F, Potenza N, Chioccarelli T, Di Palo A, Siniscalchi C, Porreca V, Scialla A, Mele VG, Petito G, Russo A, Lanni A, Senese R, Ricci G, Pierantoni R, Chianese R, Cobellis G. Actin remodeling driven by circLIMA1: sperm cell as an intriguing cellular model. Int J Biol Sci 2022; 18(13):5136-5153. doi:10.7150/ijbs.76261. https://www.ijbs.com/v18p5136.htm
CSE
Manfrevola F, Potenza N, Chioccarelli T, Di Palo A, Siniscalchi C, Porreca V, Scialla A, Mele VG, Petito G, Russo A, Lanni A, Senese R, Ricci G, Pierantoni R, Chianese R, Cobellis G. 2022. Actin remodeling driven by circLIMA1: sperm cell as an intriguing cellular model. Int J Biol Sci. 18(13):5136-5153.
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