Cloning, characterization, and expression analysis of goat (<i>Capra hircus</i>) phospholipid hydroperoxide glutathione peroxidase (PHGPx)

SHI, Li-guang; XUN, Wen-juan; YUE, Wen-bin; ZHANG, Chun-xiang; REN, You-she; WANG, Qian; WU, Xiao-ying; SHI, Lei; YANG, Ru-jie; LEI, Fu-lin

doi:10.7150/ijbs.6.316

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Int J Biol Sci 2010; 6(4):316-326. doi:10.7150/ijbs.6.316 This issue Cite

Research Paper

Cloning, characterization, and expression analysis of goat (Capra hircus) phospholipid hydroperoxide glutathione peroxidase (PHGPx)

Li-guang SHI¹, Wen-juan XUN¹, Wen-bin YUE¹^✉, Chun-xiang ZHANG¹, You-she REN¹, Qian WANG¹, Xiao-ying WU¹, Lei SHI¹, Ru-jie YANG^{1, 2}, Fu-lin LEI³

1. College of Animal Science and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi 030801, China
2. College of Animal Science and Veterinary Medicine; Taiyuan Shanxi 030024, China
3. Licheng sheep Breeding Base of Shanxi Agricultural University, Licheng Shanxi 047600, China

Citation:

SHI Lg, XUN Wj, YUE Wb, ZHANG Cx, REN Ys, WANG Q, WU Xy, SHI L, YANG Rj, LEI Fl. Cloning, characterization, and expression analysis of goat (Capra hircus) phospholipid hydroperoxide glutathione peroxidase (PHGPx). Int J Biol Sci 2010; 6(4):316-326. doi:10.7150/ijbs.6.316. https://www.ijbs.com/v06p0316.htm

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Abstract

Phospholipid hydroperoxide glutathione peroxidase (PHGPx), as a ubiquitous antioxidant enzyme in the glutathione peroxidases (GPx) family, plays multiple roles in organisms. However, there is very little information on PHGPx in goats (Capra hircus). In this study, a full-length cDNA was cloned and characterized from Taihang black goat testes. The 844 bp cDNA contains an open reading frame (ORF) of 597 bp. The goat PHGPx nucleotide sequence contains a selenocysteine (sec) codon TGA^244-246, two potential start codons ATG^20-22 and ATG^108-110, a polyadenylation signal AATAAA^813-818 and selenocysteine insertion sequence (SECIS) motif AUGA^688-691, UGA^729-731 and AAA^703-705. As a selenoprotein, the active-site motifs and GPx family signature motifs LAFPCNQF^101-108 and WNFEK^165-170 were also found. The order of PHGPx mRNA expression levels was: testes >> heart > brain > epididymis > kidney > liver > lung > spleen > muscle. Real-time PCR and immunohistochemistry results revealed similar expression differences in different age testes, with high expression levels during adolescence. Immunofluorescence results suggested that PHGPx mainly expressed in Leydig cells and spermatids in mature goat testes.

Keywords: goat, phospholipid hydroperoxide glutathione peroxidase, real-time PCR, expression, immunohistochemistry.

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APA

SHI, L.g., XUN, W.j., YUE, W.b., ZHANG, C.x., REN, Y.s., WANG, Q., WU, X.y., SHI, L., YANG, R.j., LEI, F.l. (2010). Cloning, characterization, and expression analysis of goat (Capra hircus) phospholipid hydroperoxide glutathione peroxidase (PHGPx). International Journal of Biological Sciences, 6(4), 316-326. https://doi.org/10.7150/ijbs.6.316.

ACS

SHI, L.g.; XUN, W.j.; YUE, W.b.; ZHANG, C.x.; REN, Y.s.; WANG, Q.; WU, X.y.; SHI, L.; YANG, R.j.; LEI, F.l. Cloning, characterization, and expression analysis of goat (Capra hircus) phospholipid hydroperoxide glutathione peroxidase (PHGPx). Int. J. Biol. Sci. 2010, 6 (4), 316-326. DOI: 10.7150/ijbs.6.316.

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CSE

SHI Lg, XUN Wj, YUE Wb, ZHANG Cx, REN Ys, WANG Q, WU Xy, SHI L, YANG Rj, LEI Fl. 2010. Cloning, characterization, and expression analysis of goat (Capra hircus) phospholipid hydroperoxide glutathione peroxidase (PHGPx). Int J Biol Sci. 6(4):316-326.

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