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Int J Biol Sci 2016; 12(10):1213-1224. doi:10.7150/ijbs.15496 This issue Cite

Research Paper

miR-628 Promotes Burn-Induced Skeletal Muscle Atrophy via Targeting IRS1

Yonghui Yu*, Xiao Li*, Lingying Liu*, Jiake Chai✉, Zhang Haijun, Wanli Chu, Huinan Yin, Li Ma, Hongjie Duan, Mengjing Xiao

Department of Burn & Plastic Surgery, the First Affiliated Hospital of PLA General Hospital, Beijing, 100048 China.
*These authors contributed equally to this work.

✉ Corresponding author: Chai Jiake, Ph.D. Department of Burn & Plastic Surgery, the First Affiliated Hospital of PLA General Hospital, 51 Fucheng Road, Haidian District, Beijing, 100048 China. Tel: +86-10-66867972 Fax: +86-10-68989181 Email: cjk30 More

Citation:
Yu Y, Li X, Liu L, Chai J, Haijun Z, Chu W, Yin H, Ma L, Duan H, Xiao M. miR-628 Promotes Burn-Induced Skeletal Muscle Atrophy via Targeting IRS1. Int J Biol Sci 2016; 12(10):1213-1224. doi:10.7150/ijbs.15496. https://www.ijbs.com/v12p1213.htm
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Abstract

Graphic abstract

Skeletal muscle atrophy is a common clinical feature among patients with severe burns. Previous studies have shown that miRNAs play critical roles in the regulation of stress-induced skeletal muscle atrophy. Our previous study showed that burn-induced skeletal muscle atrophy is mediated by miR-628. In this study, compared with sham rats, rats subjected to burn injury exhibited skeletal muscle atrophy, as well as significantly decreased insulin receptor substrate 1 (IRS1) protein expression and significantly increased skeletal muscle cell apoptosis. An miRNA array showed that the levels of miR-628, a potential regulator of IRS1 protein translation, were also clearly elevated. Second, L6 myocyte cell apoptosis increased after induction of miR-628 expression, and IRS1 and p-Akt protein expression decreased significantly. Expression of the cell apoptosis-related proteins FoxO3a and cleaved caspase 3 also increased after induction of miR-628 expression. Finally, forced miR-628 expression in normal rats resulted in increased cell apoptosis and skeletal muscle atrophy, as well as changes in IRS1/Akt/FoxO3a signaling pathway activity consistent with the changes in protein expression described above. Inhibiting cell apoptosis with Z-VAD-FMK resulted in alleviation of burn-induced skeletal muscle atrophy. In general, our results indicate that miR-628 mediates burn-induced skeletal muscle atrophy by regulating the IRS1/Akt/FoxO3a signaling pathway.

Keywords: miR-628, Burn, IRS1, Skeletal muscle atrophy.

Citation styles

APA
Yu, Y., Li, X., Liu, L., Chai, J., Haijun, Z., Chu, W., Yin, H., Ma, L., Duan, H., Xiao, M. (2016). miR-628 Promotes Burn-Induced Skeletal Muscle Atrophy via Targeting IRS1. International Journal of Biological Sciences, 12(10), 1213-1224. https://doi.org/10.7150/ijbs.15496.

ACS
Yu, Y.; Li, X.; Liu, L.; Chai, J.; Haijun, Z.; Chu, W.; Yin, H.; Ma, L.; Duan, H.; Xiao, M. miR-628 Promotes Burn-Induced Skeletal Muscle Atrophy via Targeting IRS1. Int. J. Biol. Sci. 2016, 12 (10), 1213-1224. DOI: 10.7150/ijbs.15496.

NLM
Yu Y, Li X, Liu L, Chai J, Haijun Z, Chu W, Yin H, Ma L, Duan H, Xiao M. miR-628 Promotes Burn-Induced Skeletal Muscle Atrophy via Targeting IRS1. Int J Biol Sci 2016; 12(10):1213-1224. doi:10.7150/ijbs.15496. https://www.ijbs.com/v12p1213.htm

CSE
Yu Y, Li X, Liu L, Chai J, Haijun Z, Chu W, Yin H, Ma L, Duan H, Xiao M. 2016. miR-628 Promotes Burn-Induced Skeletal Muscle Atrophy via Targeting IRS1. Int J Biol Sci. 12(10):1213-1224.

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