MiR-519d suppresses breast cancer tumorigenesis and metastasis via targeting MMP3

Chu, Chengling; Liu, Xin; Bai, Xue; Zhao, Tong; Wang, Mengxue; Xu, Ranchen; Li, Mingqi; Hu, Yingying; Li, Weihua; Yang, Lida; Qin, Youyou; Yang, Meng; Yan, Chaoqi; Zhang, Yong

doi:10.7150/ijbs.22849

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International Journal of Medical Sciences

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Int J Biol Sci 2018; 14(2):228-236. doi:10.7150/ijbs.22849 This issue Cite

Research Paper

MiR-519d suppresses breast cancer tumorigenesis and metastasis via targeting MMP3

Chengling Chu¹, Xin Liu², Xue Bai², Tong Zhao², Mengxue Wang², Ranchen Xu², Mingqi Li², Yingying Hu³, Weihua Li³, Lida Yang², Youyou Qin⁴, Meng Yang¹, Chaoqi Yan^4✉, Yong Zhang^2,5✉

1. Department of Oncology, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China;
2. Department of Pharmacology (the State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin 150081, China;
3. Department of Pharmacy, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China;
4. Department of Breast Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China;
5. Institute of Metabolic Disease, Heilongjiang Academy of Medical Science, Harbin 150086, China

Citation:

Chu C, Liu X, Bai X, Zhao T, Wang M, Xu R, Li M, Hu Y, Li W, Yang L, Qin Y, Yang M, Yan C, Zhang Y. MiR-519d suppresses breast cancer tumorigenesis and metastasis via targeting MMP3. Int J Biol Sci 2018; 14(2):228-236. doi:10.7150/ijbs.22849. https://www.ijbs.com/v14p0228.htm

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Abstract

Breast cancer (BC) is the most common cause of death in women throughout the world. Although microRNAs (miRNAs) have been identified as novel regulators in carcinogenesis, there are still abundant hidden treasure needed to be excavated. In the present study, we found that miR-519d expression was remarkably decreased in both human BC tissues and MCF-7 cells. CCK8 and 5-Ethynyl-2′-deoxyuridine (EdU) assays were used to evaluate cell proliferation. Wound-healing and transwell assays were performed for detection of cell migration and invasion. The results demonstrated miR-519d overexpression dramatically suppressed MCF-7 cells proliferation, migration and invasion. While downregulation of miR-519d by miR-519d inhibitor substantially increased MCF-7 cell carcinogenesis. Further analysis identified Matrix Metalloproteinase-3 (MMP3) as a direct target of miR-519d. QRT-PCR and western blot results indicated the correlative expression of miR-519d and MMP3 in BC tissues and MCF-7 cells. In summary, our data uncovered the novel molecular interaction between miR-519d and MMP3, indicating a therapeutic strategy of miR-519d for BC.

Keywords: breast cancer, metastasis, miR-519d, MMP3

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APA

Chu, C., Liu, X., Bai, X., Zhao, T., Wang, M., Xu, R., Li, M., Hu, Y., Li, W., Yang, L., Qin, Y., Yang, M., Yan, C., Zhang, Y. (2018). MiR-519d suppresses breast cancer tumorigenesis and metastasis via targeting MMP3. International Journal of Biological Sciences, 14(2), 228-236. https://doi.org/10.7150/ijbs.22849.

ACS

Chu, C.; Liu, X.; Bai, X.; Zhao, T.; Wang, M.; Xu, R.; Li, M.; Hu, Y.; Li, W.; Yang, L.; Qin, Y.; Yang, M.; Yan, C.; Zhang, Y. MiR-519d suppresses breast cancer tumorigenesis and metastasis via targeting MMP3. Int. J. Biol. Sci. 2018, 14 (2), 228-236. DOI: 10.7150/ijbs.22849.

NLM

CSE

Chu C, Liu X, Bai X, Zhao T, Wang M, Xu R, Li M, Hu Y, Li W, Yang L, Qin Y, Yang M, Yan C, Zhang Y. 2018. MiR-519d suppresses breast cancer tumorigenesis and metastasis via targeting MMP3. Int J Biol Sci. 14(2):228-236.

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