Successful Spermatogonial Stem Cells Transplantation within Pleuronectiformes: First Breakthrough at inter-family Level in Marine Fish

Zhou, Li; Wang, Xueying; Liu, Qinghua; Yang, Jingkun; Xu, Shihong; Wu, Zhihao; Wang, Yanfeng; You, Feng; Song, Zongcheng; Li, Jun

doi:10.7150/ijbs.63266

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Int J Biol Sci 2021; 17(15):4426-4441. doi:10.7150/ijbs.63266 This issue Cite

Research Paper

Successful Spermatogonial Stem Cells Transplantation within Pleuronectiformes: First Breakthrough at inter-family Level in Marine Fish

Li Zhou^1,2,3*, Xueying Wang^1,2*, Qinghua Liu^1,2✉, Jingkun Yang¹, Shihong Xu^1,2, Zhihao Wu¹, Yanfeng Wang^1,2, Feng You¹, Zongcheng Song⁴, Jun Li^1,2✉

1. The Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.
2. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
3. College of Life Science, Ningde Normal University, Engineering Research Center of Mindong Aquatic Product Deep-Processing, Fujian Province University, Ningde, China.
4. Weihai Shenghang Aquatic Product Science and Technology Co. Ltd., Weihai, China.
*These authors contributed equally to this work.

Citation:

Zhou L, Wang X, Liu Q, Yang J, Xu S, Wu Z, Wang Y, You F, Song Z, Li J. Successful Spermatogonial Stem Cells Transplantation within Pleuronectiformes: First Breakthrough at inter-family Level in Marine Fish. Int J Biol Sci 2021; 17(15):4426-4441. doi:10.7150/ijbs.63266. https://www.ijbs.com/v17p4426.htm

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Abstract

As a promising biotechnology, fish germ cell transplantation shows potentials in conservation germplasm resource, propagation of elite species, and generation of transgenic individuals. In this study, we successfully transplanted the Japanese flounder (P. olivaceus), summer flounder (P. dentatus), and turbot (S. maximus) spermatogonia into triploid Japanese flounder larvae, and achieved high transplantation efficiency of 100%, 75-95% and 33-50% by fluorescence tracking and molecular analysis, respectively. Eventually, donor-derived spermatozoa produced offspring by artificial insemination. We only found male and intersex chimeras in inter-family transplantations, while male and female chimeras in both intra-species and intra-genus transplantations. Moreover, the intersex chimeras could mature and produce turbot functional spermatozoa. We firstly realized inter-family transplantation in marine fish species. These results demonstrated successful spermatogonial stem cells transplantation within Pleuronectiformes, suggesting the germ cells migration, incorporation and maturation within order were conserved across a wide range of teleost species.

Keywords: Pleuronectiformes, germ cell transplantation, spermatogonia, triploid recipient, inter-family

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APA

Zhou, L., Wang, X., Liu, Q., Yang, J., Xu, S., Wu, Z., Wang, Y., You, F., Song, Z., Li, J. (2021). Successful Spermatogonial Stem Cells Transplantation within Pleuronectiformes: First Breakthrough at inter-family Level in Marine Fish. International Journal of Biological Sciences, 17(15), 4426-4441. https://doi.org/10.7150/ijbs.63266.

ACS

Zhou, L.; Wang, X.; Liu, Q.; Yang, J.; Xu, S.; Wu, Z.; Wang, Y.; You, F.; Song, Z.; Li, J. Successful Spermatogonial Stem Cells Transplantation within Pleuronectiformes: First Breakthrough at inter-family Level in Marine Fish. Int. J. Biol. Sci. 2021, 17 (15), 4426-4441. DOI: 10.7150/ijbs.63266.

NLM

CSE

Zhou L, Wang X, Liu Q, Yang J, Xu S, Wu Z, Wang Y, You F, Song Z, Li J. 2021. Successful Spermatogonial Stem Cells Transplantation within Pleuronectiformes: First Breakthrough at inter-family Level in Marine Fish. Int J Biol Sci. 17(15):4426-4441.

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