ISSN: 1449-2288International Journal of Biological Sciences
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Int J Biol Sci 2022; 18(2):731-741. doi:10.7150/ijbs.64956 This issue Cite
Research Paper
ATPase inhibitory factor 1 protects the heart from acute myocardial ischemia/reperfusion injury through activating AMPK signaling pathway
Department of Cardiology, The First Affiliated Hospital of USTC, Division of Life Science and Medicine, University of Science and Technology of China, Hefei, 230001, China
Citation:
Wu JW, Hu H, Hua Js, Ma LK. ATPase inhibitory factor 1 protects the heart from acute myocardial ischemia/reperfusion injury through activating AMPK signaling pathway. Int J Biol Sci 2022; 18(2):731-741. doi:10.7150/ijbs.64956. https://www.ijbs.com/v18p0731.htm
Other stylesAbstract
Rationale: Myocardial ischemia/reperfusion (I/R) injury is a common clinic scenario that occurs in the context of reperfusion therapy for acute myocardial infarction (AMI). The mitochondrial F1Fo-ATPase inhibitory factor 1 (IF1) blocks the reversal of the F1Fo-ATP synthase to prevent detrimental consumption of cellular ATP and associated demise. In the present study, we study the role and mechanism of IF1 in myocardial I/R injury.
Methods: Mice were ligated the left anterior descending coronary artery to build the I/R model in vivo. Rat hearts were isolated and perfused with constant pressure according to Langendorff. Also, neonatal cardiomyocytes hypoxia-reoxygenation (H/R) model was also used. Myocardial infarction area, cardiac function, cellular function, and cell viability was conducted and compared.
Results: Our data revealed that IF1 is upregulated in hearts after I/R and cardiomyocytes with hypoxia/re-oxygenation (H/R). IF1 delivered with adenovirus and adeno-associated virus serotype 9 (AAV9) ameliorated cardiac dysfunction and pathological development induced by I/R ex vivo and in vivo. Mechanistically, IF1 stimulates glucose uptake and glycolysis activity and stimulates AMPK activation during in vivo basal and I/R and in vitro OGD/R conditions, and activation of AMPK by IF1 is responsible for its cardioprotective effects against H/R-induced injury.
Conclusions: These results suggest that increased IF1 in the I/R heart confer cardioprotective effects via activating AMPK signaling. Therefore, IF1 can be used as a potential therapeutic target for the treatment of pathological ischemic injury and heart failure.
Keywords: ATPase inhibitory factor 1, ischemia/reperfusion injury, heart, AMPK
Citation styles
APA
Wu, J.W., Hu, H., Hua, J.s., Ma, L.K. (2022). ATPase inhibitory factor 1 protects the heart from acute myocardial ischemia/reperfusion injury through activating AMPK signaling pathway. International Journal of Biological Sciences, 18(2), 731-741. https://doi.org/10.7150/ijbs.64956.
ACS
Wu, J.W.; Hu, H.; Hua, J.s.; Ma, L.K. ATPase inhibitory factor 1 protects the heart from acute myocardial ischemia/reperfusion injury through activating AMPK signaling pathway. Int. J. Biol. Sci. 2022, 18 (2), 731-741. DOI: 10.7150/ijbs.64956.
NLM
Wu JW, Hu H, Hua Js, Ma LK. ATPase inhibitory factor 1 protects the heart from acute myocardial ischemia/reperfusion injury through activating AMPK signaling pathway. Int J Biol Sci 2022; 18(2):731-741. doi:10.7150/ijbs.64956. https://www.ijbs.com/v18p0731.htm
CSE
Wu JW, Hu H, Hua Js, Ma LK. 2022. ATPase inhibitory factor 1 protects the heart from acute myocardial ischemia/reperfusion injury through activating AMPK signaling pathway. Int J Biol Sci. 18(2):731-741.
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