ISSN: 1449-2288International Journal of Biological Sciences
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Int J Biol Sci 2022; 18(15):5809-5826. doi:10.7150/ijbs.77734 This issue Cite
Research Paper
Biologically synthesis of gold nanoparticles using Cirsium japonicum var. maackii extract and the study of anti-cancer properties on AGS gastric cancer cells
1. Graduate School of Biotechnology, and College of Life Science, Kyung Hee University, Yongin-si, 17104, Gyeonggi-do, Republic of Korea.
2. Department of Plant Science and Technology, Chung-Ang University, Anseong 17546, Republic of Korea.
Citation:
Mi Xj, Park HR, Dhandapani S, Lee S, Kim YJ. Biologically synthesis of gold nanoparticles using Cirsium japonicum var. maackii extract and the study of anti-cancer properties on AGS gastric cancer cells. Int J Biol Sci 2022; 18(15):5809-5826. doi:10.7150/ijbs.77734. https://www.ijbs.com/v18p5809.htm
Other stylesAbstract
Plant extract-mediated synthesis of metal nanoparticles (NPs) is an eco-friendly and cost-effective biosynthesis method that is more suitable for biological applications than chemical ones. We prepared novel gold NPs (AuNPs), Cirsium japonicum mediated-AuNPs (CJ-AuNPs), using a biosynthetic process involving Cirsium japonicum (Herba Cirsii, CJ) ethanol extract. The physicochemical properties of CJ-AuNPs were characterized using spectrometric and microscopic analyses. The in vitro stability of CJ-AuNPs was studied for 3 months. Moreover, the selective human gastric adenocarcinoma (AGS) cell killing ability of CJ-AuNPs was verified in cancer and normal cells. An in vitro study revealed that CJ-AuNPs trigger oxidative stress and iron-dependent ferroptosis in AGS cells. Mechanistically, CJ-AuNPs induced mitochondrial reactive oxygen species (ROS), Fe2+, and lipid peroxidation accumulation, and mitochondrial damage by destroying the glutathione peroxidase-4 (GPX4)-dependent antioxidant capacity. Furthermore, in a xenograft mouse model implanted with AGS cells, treatment with 2.5, 5, and 10 mg/kg CJ-AuNPs for 16 days reduced tumor xenograft growth in a dose dependent manner in vivo without systemic toxicity. These results demonstrate that CJ-AuNPs exert anticancer effects in vitro and in vivo by inducing ferroptosis-mediated cancer cell death. This study, based on green-synthesized nanodrug-induced ferroptosis, provides new insight into potential developments in cancer therapies.
Keywords: Cirsium japonicum, Gold nanoparticles, Gastric cancer, Ferroptosis
Citation styles
APA
Mi, X.j., Park, H.R., Dhandapani, S., Lee, S., Kim, Y.J. (2022). Biologically synthesis of gold nanoparticles using Cirsium japonicum var. maackii extract and the study of anti-cancer properties on AGS gastric cancer cells. International Journal of Biological Sciences, 18(15), 5809-5826. https://doi.org/10.7150/ijbs.77734.
ACS
Mi, X.j.; Park, H.R.; Dhandapani, S.; Lee, S.; Kim, Y.J. Biologically synthesis of gold nanoparticles using Cirsium japonicum var. maackii extract and the study of anti-cancer properties on AGS gastric cancer cells. Int. J. Biol. Sci. 2022, 18 (15), 5809-5826. DOI: 10.7150/ijbs.77734.
NLM
Mi Xj, Park HR, Dhandapani S, Lee S, Kim YJ. Biologically synthesis of gold nanoparticles using Cirsium japonicum var. maackii extract and the study of anti-cancer properties on AGS gastric cancer cells. Int J Biol Sci 2022; 18(15):5809-5826. doi:10.7150/ijbs.77734. https://www.ijbs.com/v18p5809.htm
CSE
Mi Xj, Park HR, Dhandapani S, Lee S, Kim YJ. 2022. Biologically synthesis of gold nanoparticles using Cirsium japonicum var. maackii extract and the study of anti-cancer properties on AGS gastric cancer cells. Int J Biol Sci. 18(15):5809-5826.
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