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Int J Biol Sci 2023; 19(5):1645-1663. doi:10.7150/ijbs.81939 This issue Cite

Research Paper

Novel Implication of the Basement Membrane for Breast Cancer Outcome and Immune Infiltration

Wenwen Tian1,†, Yongzhou Luo1,†, Yuhui Tang1,†, Yanan Kong1†, Linyu Wu1, Shaoquan Zheng2, Yutian Zou1, Chao Zhang1, Jindong Xie1, Xinpei Deng1, Junsheng Zhang1, Nian Chen3,✉, Xiaoming Xie1,✉, Weidong Wei1,✉

1. State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, Collaborative Innovation Center for Cancer Medicine, 651 East Dongfeng Road, Guangzhou, 510060, China.
2. Breast Disease Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, People's Republic of China.
3. The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, 421001, China.
† These authors contributed equally to this work

✉ Corresponding authors: Weidong Wei (weiwdorg.cn), Xiaoming Xie (xiexmorg.cn): State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, Collaborative Innovation Center for Cancer Medicine, 651 East Dongfe More

Citation:
Tian W, Luo Y, Tang Y, Kong Y, Wu L, Zheng S, Zou Y, Zhang C, Xie J, Deng X, Zhang J, Chen N, Xie X, Wei W. Novel Implication of the Basement Membrane for Breast Cancer Outcome and Immune Infiltration. Int J Biol Sci 2023; 19(5):1645-1663. doi:10.7150/ijbs.81939. https://www.ijbs.com/v19p1645.htm
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Abstract

Graphic abstract

Therapeutic failure in breast cancer patients is largely attributed to postoperative advancement and therapy resistance. Nevertheless, an efficacious prognostic signature for recognizing this population is lacking. The basement membrane (BM) has been proven to be strongly involved in cancer progression and metastasis, and has the potential to be a powerful predictor in breast cancer. In this study, substantial bulk RNA transcriptomics, single cell RNA transcriptomics and clinical information were collected from TCGA-BRCA, METABRIC and GSE96058, and Kaplan-Meier survival curves, single cell analysis and in vitro experiments were conducted to validate the signature. From the results, a prognostic index, namely, the BMscore, was established with six pivotal BM genes, specifically LOXL1, FBLN1, FBLN5, SDC1, ADAMTS8 and PXDNL. Verification by independent cohorts showed that breast cancer patients with high BMscore had a distinctly worse outcome. By integrating the BMscore and clinical factors, we constructed a prognostic nomogram that displayed good predictive capability. Furthermore, we evaluated the implication of the BMscore in breast cancer immune infiltration. More importantly, a strongly positive correlation between the BMscore and EMT activity was revealed with immunohistochemistry and in vitro experiments. Taken together, we provided a novel BMscore gene signature for breast cancer patients to predict clinical prognosis and metastasis accurately, which may help with individualized clinical decision-making.

Keywords: breast cancer, prognosis, basement membrane, immune infiltration.

Citation styles

APA
Tian, W., Luo, Y., Tang, Y., Kong, Y., Wu, L., Zheng, S., Zou, Y., Zhang, C., Xie, J., Deng, X., Zhang, J., Chen, N., Xie, X., Wei, W. (2023). Novel Implication of the Basement Membrane for Breast Cancer Outcome and Immune Infiltration. International Journal of Biological Sciences, 19(5), 1645-1663. https://doi.org/10.7150/ijbs.81939.

ACS
Tian, W.; Luo, Y.; Tang, Y.; Kong, Y.; Wu, L.; Zheng, S.; Zou, Y.; Zhang, C.; Xie, J.; Deng, X.; Zhang, J.; Chen, N.; Xie, X.; Wei, W. Novel Implication of the Basement Membrane for Breast Cancer Outcome and Immune Infiltration. Int. J. Biol. Sci. 2023, 19 (5), 1645-1663. DOI: 10.7150/ijbs.81939.

NLM
Tian W, Luo Y, Tang Y, Kong Y, Wu L, Zheng S, Zou Y, Zhang C, Xie J, Deng X, Zhang J, Chen N, Xie X, Wei W. Novel Implication of the Basement Membrane for Breast Cancer Outcome and Immune Infiltration. Int J Biol Sci 2023; 19(5):1645-1663. doi:10.7150/ijbs.81939. https://www.ijbs.com/v19p1645.htm

CSE
Tian W, Luo Y, Tang Y, Kong Y, Wu L, Zheng S, Zou Y, Zhang C, Xie J, Deng X, Zhang J, Chen N, Xie X, Wei W. 2023. Novel Implication of the Basement Membrane for Breast Cancer Outcome and Immune Infiltration. Int J Biol Sci. 19(5):1645-1663.

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