Int J Biol Sci 2014; 10(1):43-52. doi:10.7150/ijbs.6818 This issue

Research Paper

Important Functional Roles of Basigin in Thymocyte Development and T cell Activation

Hui Yao1*, Yan Teng2*, Qian Sun1, Jing Xu1, Ya-Tong Chen1, Ning Hou2, Xuan Cheng2, Xiao Yang2✉, Zhi-Nan Chen1✉

1. Cell Engineering Research Centre & Department of Cell Biology, State Key Laboratory of Cancer Biology, Fourth Military Medical University, 169 West Changle Street, Xi'an 710032, China.
2. Genetic Laboratory of Development and Disease, Institute of Biotechnology, 20 Dongdajie, Fengtai District, Beijing 100071, China.
* These authors contributed equally to this work.

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Yao H, Teng Y, Sun Q, Xu J, Chen YT, Hou N, Cheng X, Yang X, Chen ZN. Important Functional Roles of Basigin in Thymocyte Development and T cell Activation. Int J Biol Sci 2014; 10(1):43-52. doi:10.7150/ijbs.6818. Available from

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Basigin is a highly glycosylated transmembrane protein that is expressed in a broad range of tissues and is involved in a number of physiological and pathological processes. However, the in vivo role of basigin remains unknown. To better understand the physiological and pathological functions of basigin in vivo, we generated a conditional null allele by introducing two loxP sites flanking exons 2 and 7 of the basigin gene (Bsg). Bsgfl/fl mice were born at the expected Mendelian ratio and showed a similar growth rate compared with wildtype mice. After crossing these mice with Lck-Cre transgenic mice, basigin expression was specifically inactivated in T cells in the resulting Lck-Cre; Bsgfl/fl mice. Although the birth and growth rate of Lck-Cre; Bsgfl/fl mice were similar to control mice, thymus development was partially arrested in Lck-Cre; Bsgfl/fl mice, specifically at the CD4+CD8+ double-positive (DP) and CD4 single-positive (CD4+CD8-, CD4SP) stages. In addition, CD4+ T cell activation was enhanced upon Concanavalin A (Con A) or anti-CD3/anti-CD28 stimulation but not upon PMA/Ionomycin stimulation in the absence of basigin. Overall, this study provided the first in vivo evidence for the function of basigin in thymus development. Moreover, the successful generation of the conditional null basigin allele provides a useful tool for the study of distinct physiological or pathological functions of basigin in different tissues at different development stages.

Keywords: Basigin, Cre-Loxp, gene targeting, thymocyte development, T cell activation