Int J Biol Sci
2023; 19(8):2349-2365.
doi:10.7150/ijbs.79115 This issueCite
Research Paper
Upregulated Long Non-coding RNA Lnc-MRPL39-2:1 Induces the Growth and Invasion of Nasopharyngeal Carcinoma by Binding to HuR and Stabilizing β-Catenin mRNA
Yunhong Tian1,2#, Meiling Ai1,2#, Chunshan Liu1,2#, Yuchao Wu1,2, Muhammad Khan1,2, Baiyao Wang1,2, Huidong Long1,2, Chunyue Huang1,2, Jie Lin1,2, Anan Xu1,2, Rong Li1,2, Bohong Cen1,2, Wenze Qiu1,2, Guofeng Xie1,2✉, Yawei Yuan1,2✉
1. Department of Radiation Oncology, Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou, Guangdong Province People's Republic of China 2. State Key Laboratory of Respiratory Disease, Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou China #Contributed equally.
✉ Corresponding authors: Guofeng, Xie, and Yawei Yuan, Department of Radiation Oncology, Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou, Guangdong Province, People's Republic of China. Emails: yuanyaweiedu.cn (Y.Y.), 1035124435com (G. X.)More
Citation:
Tian Y, Ai M, Liu C, Wu Y, Khan M, Wang B, Long H, Huang C, Lin J, Xu A, Li R, Cen B, Qiu W, Xie G, Yuan Y. Upregulated Long Non-coding RNA Lnc-MRPL39-2:1 Induces the Growth and Invasion of Nasopharyngeal Carcinoma by Binding to HuR and Stabilizing β-Catenin mRNA. Int J Biol Sci 2023; 19(8):2349-2365. doi:10.7150/ijbs.79115. https://www.ijbs.com/v19p2349.htm
Long non-coding RNAs (lncRNAs) have been to regulate tumor progression and therapy resistance through various molecular mechanisms. In this study, we investigated the role of lncRNAs in nasopharyngeal carcinoma (NPC) and the underlying mechanism. Using lncRNA arrays to analyze the lncRNA profiles of the NPC and para-tumor tissues, we detected the novel lnc-MRPL39-2:1, which was validated by in situ hybridization and by the 5' and 3' rapid amplification of the cDNA ends. Further, its role in NPC cell growth and metastasis was verified in vitro and in vivo. The researchers conducted the RNA pull-down assays, mass spectrometry (MS), dual-luciferase reporter assays, RNA immunoprecipitation (RIP) assays, and the MS2-RIP assays were then used to identify the lnc-MRPL39-2:1-interacting proteins and miRNAs. We found that lnc-MRPL39-2:1, which was highly expressed in in NPC tissues, was related to a poor prognosis in NPC patients. Furthermore, lnc-MRPL39-2:1 was shown to induce the growth and invasion of NPC by interacting directly with the Hu-antigen R (HuR) to upregulate β-catenin expression both in vivo and in vitro. Lnc-MRPL39-2:1 expression was also suppressed by microRNA (miR)-329. Thus, these findings indicate that lnc-MRPL39-2:1 is essential in NPC tumorigenesis and metastasis and highlight its potential as a prognostic marker and therapeutic target for NPC.
Tian, Y., Ai, M., Liu, C., Wu, Y., Khan, M., Wang, B., Long, H., Huang, C., Lin, J., Xu, A., Li, R., Cen, B., Qiu, W., Xie, G., Yuan, Y. (2023). Upregulated Long Non-coding RNA Lnc-MRPL39-2:1 Induces the Growth and Invasion of Nasopharyngeal Carcinoma by Binding to HuR and Stabilizing β-Catenin mRNA. International Journal of Biological Sciences, 19(8), 2349-2365. https://doi.org/10.7150/ijbs.79115.
ACS
Tian, Y.; Ai, M.; Liu, C.; Wu, Y.; Khan, M.; Wang, B.; Long, H.; Huang, C.; Lin, J.; Xu, A.; Li, R.; Cen, B.; Qiu, W.; Xie, G.; Yuan, Y. Upregulated Long Non-coding RNA Lnc-MRPL39-2:1 Induces the Growth and Invasion of Nasopharyngeal Carcinoma by Binding to HuR and Stabilizing β-Catenin mRNA. Int. J. Biol. Sci. 2023, 19 (8), 2349-2365. DOI: 10.7150/ijbs.79115.
NLM
Tian Y, Ai M, Liu C, Wu Y, Khan M, Wang B, Long H, Huang C, Lin J, Xu A, Li R, Cen B, Qiu W, Xie G, Yuan Y. Upregulated Long Non-coding RNA Lnc-MRPL39-2:1 Induces the Growth and Invasion of Nasopharyngeal Carcinoma by Binding to HuR and Stabilizing β-Catenin mRNA. Int J Biol Sci 2023; 19(8):2349-2365. doi:10.7150/ijbs.79115. https://www.ijbs.com/v19p2349.htm
CSE
Tian Y, Ai M, Liu C, Wu Y, Khan M, Wang B, Long H, Huang C, Lin J, Xu A, Li R, Cen B, Qiu W, Xie G, Yuan Y. 2023. Upregulated Long Non-coding RNA Lnc-MRPL39-2:1 Induces the Growth and Invasion of Nasopharyngeal Carcinoma by Binding to HuR and Stabilizing β-Catenin mRNA. Int J Biol Sci. 19(8):2349-2365.
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