Identification of Tmem10 as a Novel Late-stage Oligodendrocytes Marker for Detecting Hypomyelination

Jiang, Wanxiang; Yang, Wanchun; Yang, Weiwei; Zhang, Junyan; Pang, Dejiang; Gan, Lingxue; Luo, Liping; Fan, Yingjun; Liu, Yanhui; Chen, Mina

doi:10.7150/ijbs.7526

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Int J Biol Sci 2014; 10(1):33-42. doi:10.7150/ijbs.7526 This issue Cite

Research Paper

Identification of Tmem10 as a Novel Late-stage Oligodendrocytes Marker for Detecting Hypomyelination

Wanxiang Jiang^1#, Wanchun Yang^1#, Weiwei Yang¹, Junyan Zhang¹, Dejiang Pang¹, Lingxue Gan¹, Liping Luo¹, Yingjun Fan², Yanhui Liu^2✉, Mina Chen^1✉

1. State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
2. Department of neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, China.
# These authors contributed equally to this paper

Citation:

Jiang W, Yang W, Yang W, Zhang J, Pang D, Gan L, Luo L, Fan Y, Liu Y, Chen M. Identification of Tmem10 as a Novel Late-stage Oligodendrocytes Marker for Detecting Hypomyelination. Int J Biol Sci 2014; 10(1):33-42. doi:10.7150/ijbs.7526. https://www.ijbs.com/v10p0033.htm

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Abstract

Oligodendrocytes ensheath axons to form compact insulating multilamellar structures known as myelin. Tmem10 is a novel type I transmembrane glycoprotein that is highly expressed in oligodendrocytes and whose biological function remains largely unknown. Furthermore, the expression pattern of Tmem10 remains a matter of some controversy. Given the inconsistency of its expression pattern and the lack of validated specific antibodies, Tmem10 is not widely accepted as a marker for mature oligodendrocytes. As a means to solve these problems and to aid future studies of oligodendrocyte-associated diseases, we have generated a highly specific Tmem10 antibody. Using this Tmem10 antibody, we clarify that Tmem10 protein is firstly expressed at 2 weeks in the postnatal mouse brain with age-related increase, only in the central nervous system (CNS). We also reveal that Tmem10 is expressed specifically in late stage oligodendrocytes and later than MAG, a late-stage myelin marker. Finally, we show that Tmem10 co-expresses with MOG- and MBP-positive myelin fibers and is dramatically reduced in a hypomyelination mouse model. In conclusion, our study demonstrates that Tmem10 can be used as a specific marker for myelinating oligodendrocytes and perhaps for the evaluation of myelination diseases, such as multiple sclerosis.

Keywords: Tmem10, polyclonal antibody, late stage oligodendrocytes maker, Myelination

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APA

Jiang, W., Yang, W., Yang, W., Zhang, J., Pang, D., Gan, L., Luo, L., Fan, Y., Liu, Y., Chen, M. (2014). Identification of Tmem10 as a Novel Late-stage Oligodendrocytes Marker for Detecting Hypomyelination. International Journal of Biological Sciences, 10(1), 33-42. https://doi.org/10.7150/ijbs.7526.

ACS

Jiang, W.; Yang, W.; Yang, W.; Zhang, J.; Pang, D.; Gan, L.; Luo, L.; Fan, Y.; Liu, Y.; Chen, M. Identification of Tmem10 as a Novel Late-stage Oligodendrocytes Marker for Detecting Hypomyelination. Int. J. Biol. Sci. 2014, 10 (1), 33-42. DOI: 10.7150/ijbs.7526.

NLM

CSE

Jiang W, Yang W, Yang W, Zhang J, Pang D, Gan L, Luo L, Fan Y, Liu Y, Chen M. 2014. Identification of Tmem10 as a Novel Late-stage Oligodendrocytes Marker for Detecting Hypomyelination. Int J Biol Sci. 10(1):33-42.

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