SAK-HV Promotes RAW264.7 cells Migration Mediated by MCP-1 via JNK and NF-κB Pathways

Chen, Yao; Fu, Wen-Liang; Gan, Xiang-Dong; Xing, Wei-Wei; Xia, Wen-Rong; Zou, Min-Ji; Liu, Qing; Wang, Yuan-Yuan; Zhang, Chao; Xu, Dong-Gang

doi:10.7150/ijbs.27459

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Int J Biol Sci 2018; 14(14):1993-2002. doi:10.7150/ijbs.27459 This issue Cite

Research Paper

SAK-HV Promotes RAW264.7 cells Migration Mediated by MCP-1 via JNK and NF-κB Pathways

Yao Chen¹, Wen-Liang Fu¹, Xiang-Dong Gan¹, Wei-Wei Xing¹, Wen-Rong Xia¹, Min-Ji Zou¹, Qing Liu¹, Yuan-Yuan Wang¹, Chao Zhang^2✉, Dong-Gang Xu^1✉

1. Institute of Military Cognitive and Brain Sciences, Beijing, 100850, China.
2. Division of Oncology Research, Mayo Clinic, Rochester, MN 55905, USA.

Citation:

Chen Y, Fu WL, Gan XD, Xing WW, Xia WR, Zou MJ, Liu Q, Wang YY, Zhang C, Xu DG. SAK-HV Promotes RAW264.7 cells Migration Mediated by MCP-1 via JNK and NF-κB Pathways. Int J Biol Sci 2018; 14(14):1993-2002. doi:10.7150/ijbs.27459. https://www.ijbs.com/v14p1993.htm

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Abstract

Macrophage migration plays an essential role in immune system and is also involved in many pathological situations. However, the regulatory mechanism of macrophage migration remains to be elucidated due to its diverse responses to various stimuli. SAK-HV, a multifunctional protein possessing thrombolytic and lipid-lowering activity, can selectively induce the macrophage proliferation. Here, we reported SAK-HV significantly triggered RAW264.7 cells migration through its functional domain of SAK-mutant by activating both c-jun N-terminal kinases (JNK) and nuclear factor-κB (NF-κB) pathways. Meanwhile, SAK-HV upregulated the expression of some effector proteins, among which only the expression of Monocyte chemoattractant protein-1 (MCP-1) was inhibited by the blockade of JNK and NF-κB pathways. Further research showed that MCP-1 promoted migration ultimately by interacting with Chemokine (C-C motif) Receptor 2 (CCR2) in an autocrine manner. In summary, SAK-HV induced RAW264.7 cells migration through its SAK-mutant domain, during which MCP-1 chemokine mediated by JNK and NF-κB pathways played a key role. These results revealed a novel effect of SAK-HV on modulating macrophage migration and also deepened the understanding of its pharmacodynamics.

Keywords: SAK-HV, macrophage migration, MCP-1, JNK, NF-κB

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APA

Chen, Y., Fu, W.L., Gan, X.D., Xing, W.W., Xia, W.R., Zou, M.J., Liu, Q., Wang, Y.Y., Zhang, C., Xu, D.G. (2018). SAK-HV Promotes RAW264.7 cells Migration Mediated by MCP-1 via JNK and NF-κB Pathways. International Journal of Biological Sciences, 14(14), 1993-2002. https://doi.org/10.7150/ijbs.27459.

ACS

Chen, Y.; Fu, W.L.; Gan, X.D.; Xing, W.W.; Xia, W.R.; Zou, M.J.; Liu, Q.; Wang, Y.Y.; Zhang, C.; Xu, D.G. SAK-HV Promotes RAW264.7 cells Migration Mediated by MCP-1 via JNK and NF-κB Pathways. Int. J. Biol. Sci. 2018, 14 (14), 1993-2002. DOI: 10.7150/ijbs.27459.

NLM

CSE

Chen Y, Fu WL, Gan XD, Xing WW, Xia WR, Zou MJ, Liu Q, Wang YY, Zhang C, Xu DG. 2018. SAK-HV Promotes RAW264.7 cells Migration Mediated by MCP-1 via JNK and NF-κB Pathways. Int J Biol Sci. 14(14):1993-2002.

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