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Int J Biol Sci 2023; 19(7):2234-2255. doi:10.7150/ijbs.77166 This issue Cite

Research Paper

Cytochalasin D restores nuclear size acting on F-actin and IZUMO1 localization in low-quality spermatozoa

Guillaume Martinez1,2, Donato Cappetta3,4, Marialucia Telesca3, Konrad Urbanek5,6, Giuseppe Castaldo5,6, Magali Dhellemmes2, Vincenza Grazia Mele3, Teresa Chioccarelli3, Veronica Porreca3, Anne-Laure Barbotin7, Angèle Boursier7, Florian Guillou8, Charles Coutton1,2, Sophie Brouillet9, Antonella De Angelis3, Liberato Berrino3, Riccardo Pierantoni3, Gilda Cobellis3, Rosanna Chianese3✉, Francesco Manfrevola3

1. Hôpital Couple-Enfant, Centre Hospitalier Universitaire de Grenoble, UM de Génétique Chromosomique, 38000 Grenoble, France.
2. Genetic Epigenetic and Therapies of Infertility, Institute for Advanced Biosciences INSERM U1209, CNRS UMR5309, 38000 Grenoble, France.
3. Department of Experimental Medicine, University of Campania L. Vanvitelli, via Costantinopoli 16, 80138, Naples, Italy.
4. Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce.
5. Department of Molecular Medicine and Medical Biotechnologies, University of Naples “Federico II”, Via A. Pansini 5, 80131 Naples, Italy.
6. CEINGE-Advanced Biotechnologies, Via G. Salvatore 486, 80131 Naples, Italy.
7. CHU Lille, Institute de Biologie de la Reproduction-Spermiologie-CECOS, F-59000, Lille, France.
8. CNRS, IFCE, INRAE, Université de Tours, PRC, Nouzilly, France.
9. Université de Montpellier, EmbryoPluripotency, DEFE, INSERM 1203, Hôpital Arnaud de Villeneuve, CHRU Saint-Eloi, 80 Avenue Augustin Fliche, CEDEX 05, 34295 Montpellier, France.

✉ Corresponding author: Prof. Rosanna Chianese, Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", Via Costantinopoli 16, 80138, Naples, Italy. Tel. Number: +39 081 5667528; Email: rosanna.chianeseit

Citation:
Martinez G, Cappetta D, Telesca M, Urbanek K, Castaldo G, Dhellemmes M, Mele VG, Chioccarelli T, Porreca V, Barbotin AL, Boursier A, Guillou F, Coutton C, Brouillet S, De Angelis A, Berrino L, Pierantoni R, Cobellis G, Chianese R, Manfrevola F. Cytochalasin D restores nuclear size acting on F-actin and IZUMO1 localization in low-quality spermatozoa. Int J Biol Sci 2023; 19(7):2234-2255. doi:10.7150/ijbs.77166. https://www.ijbs.com/v19p2234.htm
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Abstract

Graphic abstract

In spermatozoa, the nuclear F-actin supports the acroplaxome, a subacrosomal structure involved in the correct exposure of several acrosomal membrane proteins; among them, the glycoprotein IZUMO1 is the major protein involved in sperm-oocyte fusion. Nuclear F-actin is also involved in sperm head shaping and chromosome compartmentalization.

To date, few notions regarding the bivalent role of F-actin on sperm chromatin organization and IZUMO1 positioning have been reported. In our work, we characterized subcellular organization of F-actin in human high- and low-quality spermatozoa (A- and B-SPZ), respectively, showing that F-actin over-expression in sperm head of B-SPZ affected IZUMO1 localization. A correct IZUMO1 repositioning following in vitro induction of F-actin depolymerization, by cytochalasin D treatment, occurred. Interestingly, F-actin depolymerization was also associated with a correct acrosome repositioning, thus to favor a proper acrosome reaction onset, with changes in sperm nuclear size parameters and histone acetylation rate reaching high-quality conditions.

In conclusion, the current work shows a key role of F-actin in the control of IZUMO1 localization as well as chromatin remodeling and acetylation events.

Keywords: F-actin, IZUMO1, sperm quality, chromosomes territories, histone acetylation

Citation styles

APA
Martinez, G., Cappetta, D., Telesca, M., Urbanek, K., Castaldo, G., Dhellemmes, M., Mele, V.G., Chioccarelli, T., Porreca, V., Barbotin, A.L., Boursier, A., Guillou, F., Coutton, C., Brouillet, S., De Angelis, A., Berrino, L., Pierantoni, R., Cobellis, G., Chianese, R., Manfrevola, F. (2023). Cytochalasin D restores nuclear size acting on F-actin and IZUMO1 localization in low-quality spermatozoa. International Journal of Biological Sciences, 19(7), 2234-2255. https://doi.org/10.7150/ijbs.77166.

ACS
Martinez, G.; Cappetta, D.; Telesca, M.; Urbanek, K.; Castaldo, G.; Dhellemmes, M.; Mele, V.G.; Chioccarelli, T.; Porreca, V.; Barbotin, A.L.; Boursier, A.; Guillou, F.; Coutton, C.; Brouillet, S.; De Angelis, A.; Berrino, L.; Pierantoni, R.; Cobellis, G.; Chianese, R.; Manfrevola, F. Cytochalasin D restores nuclear size acting on F-actin and IZUMO1 localization in low-quality spermatozoa. Int. J. Biol. Sci. 2023, 19 (7), 2234-2255. DOI: 10.7150/ijbs.77166.

NLM
Martinez G, Cappetta D, Telesca M, Urbanek K, Castaldo G, Dhellemmes M, Mele VG, Chioccarelli T, Porreca V, Barbotin AL, Boursier A, Guillou F, Coutton C, Brouillet S, De Angelis A, Berrino L, Pierantoni R, Cobellis G, Chianese R, Manfrevola F. Cytochalasin D restores nuclear size acting on F-actin and IZUMO1 localization in low-quality spermatozoa. Int J Biol Sci 2023; 19(7):2234-2255. doi:10.7150/ijbs.77166. https://www.ijbs.com/v19p2234.htm

CSE
Martinez G, Cappetta D, Telesca M, Urbanek K, Castaldo G, Dhellemmes M, Mele VG, Chioccarelli T, Porreca V, Barbotin AL, Boursier A, Guillou F, Coutton C, Brouillet S, De Angelis A, Berrino L, Pierantoni R, Cobellis G, Chianese R, Manfrevola F. 2023. Cytochalasin D restores nuclear size acting on F-actin and IZUMO1 localization in low-quality spermatozoa. Int J Biol Sci. 19(7):2234-2255.

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